http://localhost:80/xmlui/handle/123456789/14251 2026-02-23T22:47:44Z 2026-02-23T22:47:44Z Amara, Amro Abd-Al-Fattah http://localhost:80/xmlui/handle/123456789/16128 2023-01-20T04:37:44Z 2013-01-20T00:00:00Z

Title: Pharmaceutical and industrial protein engineering: Where we are? Authors: Amara, Amro Abd-Al-Fattah Abstract: The huge amount of information, the big number of scientists and their efforts, labs, man/hrs, fund, companies all and others factors build the success of the amazing new branch of genetic engineering the 'Protein Engineering' (PE). It concerns with the modification of protein structure/function(s) or building protein from scratch. The engineered proteins usually have new criteria(s). Engineering proteins can be mediated on the level of genes or proteins. PE fined its way in different important sectors including industrial, pharmaceutical and medicinal ones . Aspects about PE and its applications will be discussed with this review. The concept, tools, and the industrial applications of the protein, engineered proteins and PE will be under focus. In order to get up to date Knowledge about the applications of PE in basic protein and molecular biology, several examples are discussed. PE can play a significant role in different industrial and pharmaceutical sectors if used wisely and selectively.

2013-01-20T00:00:00Z Verma, Prabhakar Kumar Kamboj, Vipan Kumar http://localhost:80/xmlui/handle/123456789/16127 2023-01-20T04:34:24Z 2013-01-20T00:00:00Z

Title: HPTLC method for estimation of Olmesartan medoxomil in tablet formulation with stability studies Authors: Verma, Prabhakar Kumar; Kamboj, Vipan Kumar Abstract: A rapid resolution high performance thin layer chromatography (HPTLC) method has been developed and validated for estimation of Olmesartan medoxomil in tablet formulations. This paper describes accurate, precise, specific and reproducible method and its degradation products, related impurities for assessment of purity of bulk drug and stability of its tablet formulations. The method involve silica gel 60 F254 high performance thin layer chromatography and densitometric detection at 264 nm using toluene - acetonitrile- methanol - ethyl acetate - acetic acid (5:3.5:0.3:1:0.3 v/v/v/v). Calibration curve ranges between 300-800 ng/spot-1 Olmesartan medoxomil. Experimental design was involved forced degradation of drug, optimization of mobile phase, detection made and other chromatographic phase and study of linearity range. The total time for chromatographic separation was 6 min with a total analysis time 15 min. The proposed method was validated for its linearity, precision, recovery studies and robustness.

2013-01-20T00:00:00Z Dheeraj Baviskar Rajesh Sharma Dinesh Jain http://localhost:80/xmlui/handle/123456789/16126 2023-01-20T04:33:20Z 2013-01-20T00:00:00Z

Title: Modulation of drug release by utilizing pH-independent matrix system comprising water soluble drug verapamil hydrochloride Authors: Dheeraj Baviskar; Rajesh Sharma; Dinesh Jain Abstract: The present study was undertaken to investigate the effect of hydrophilic, plastic and hydrophobic types of polymers and their content level on the release profile of drug from matrix systems. To improve therapeutic efficacy, systemic absorption and patient compliance a sustained release matrix tablets of Verapamil HCl (VHE) has been developed. VHE tablets were prepared by using various polymers like hydrophilic (HPMC K15M CR), plastic (Kollidon SR), hydrophobic (Eudragit RSPO) and combination of best two resulted polymers using direct compression. A 32 full factorial design was applied to study the effect of polymers on drug release. For the combination of polymers, selected factors HPMC K15 CR (X1) and Eudragit RSPO (X2) showed positive influence on drug release at 18 hrs and 20 hrs. The release profile of VHE formulation exhibits Higuchi model with anomalous diffusion release. Accelerated stability trials for 3 months proved reproducibility. A good correlation between the dissolution profiles and bioavailability indicated a linear relationship between in vitro – in vivo data. The current study attained the successful design, development and optimization of controlled release once-a-day formulation of VHE.

2013-01-20T00:00:00Z Zahra Yaqeen Naqvi, Naim-ul-Hassan Hina Imran Nudrat Fatima Tehmina Sohail Zakir-ur-Rehman Atiq-ur-Rahman http://localhost:80/xmlui/handle/123456789/16121 2023-01-19T10:43:35Z 2017-07-20T00:00:00Z

Title: Evaluation of analgesic activity of P. domestica L. Authors: Zahra Yaqeen; Naqvi, Naim-ul-Hassan; Hina Imran; Nudrat Fatima; Tehmina Sohail; Zakir-ur-Rehman; Atiq-ur-Rahman Abstract: This research study was conducted to investigate acute oral toxicity and analgesic activity of ethanol extract of P. domestica fruit by using tail flick analgesiometer at 300 and 500mg/kg doses in animal models. Acute oral toxicity results showed that crude extract is safe up to the dose of 5g/kg body weight of animals. The analgesic activity revealed that P. domestica extract at 500mg/kg dose possesses highest significant and prolonged analgesic activity in dose dependent manner as compared to standard and control groups. Aspirin 300mg/kg body weight was used as standard drug. Phytochemical analysis was also carried out which showed the presence of certain phytochemicals constituents in test drug that are responsible for analgesic activity. Therefore the results are justified.

2017-07-20T00:00:00Z