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Effect of the serum of rats treated with Suo Quan pill on embryonic stem cells

2022-12-15T10:40:21Z

Title: Effect of the serum of rats treated with Suo Quan pill on embryonic stem cells Authors: Xu, Jing- Jing; Huang, Ping; Xiang, Yun-Ya; Tan, Bo; Wu, Qing-He; Ze-Hui Qin, Hong-Ying Cao Abstract: Suo Quan pill(SQP), a well-known and classical traditional Chinese medicine compound, consists of three traditional Chinese medicine: Alpinia oxyphylla Miq., Lindera aggregata (Sims) Kosterm., Dioscorea opposite. Its effect was summarized as supplementing kidney- yang and shrinkaging urination. This study evaluated the effects of the serum of rats treated with Suo Quan pill on embryonic stem cells(ES cells). Cell proliferation was detected by MTT assay. Cell cycle and apoptosis of ES cells were evaluated with flow cytometry. Nanog mRNA expression was verified by fluorescence quantitative PCR and Nanog protein in ES cells was determined by Western blot. The serum of SQP-treated rats not only promoted ES cells proliferation and Nanog expression in ES cells, but also inhibited H202 stimulated cell apoptosis. Furthermore, the serum of rats containing SQP affected the cell cycle distribution of ES cells, reducing the percentage of cells in G0/G1phase and increasing the percentage of cells in G2/M phase, increasing the proliferation index of ES cells. These results illustrate that the enhanced effect of SQP on ES cells proliferation is in part due to the increased expression of Nanog in ES cells, the accelerated cell cycle period and the inhibited apoptosis of ES cells.

Neurite outgrowth properties of Calotropis procera: In search for a neuroprotectant

2022-12-15T10:39:47Z

Title: Neurite outgrowth properties of Calotropis procera: In search for a neuroprotectant Authors: Aisha Kamal, Aisha Kamal; Zafar Saeed Saify, Zafar Saeed Saify; Khawar Saeed Jamali, Khawar Saeed Jamali; Priya Tufail, Priya Tufail; Aiman Kanwal, Aiman Kanwal; Khan, Faisal; Sonia Siddiqui, Sonia Siddiqui Abstract: In view of the well-documented medicinal properties of Calotropis procera (CP), the present study was designed to evaluate the neuroprotective effect of the extract. We have prepared a methanolic extract of Calotropis procera and screen varying concentration of CP (20, 30, 40, 50 and 70µg/ml) for the stimulatory potency on neurite outgrowth. The stimulatory effect of CP on neurite outgrowth was assessed in primary hippocampal neurons. Neurite lengths were measured using optika provison analysis software. Neuritogenesis was further analyzed by immunostaining by using specific neuronal marker β III-tubulin. The data show that neurite outgrowth from hippocampal neurons were significantly enhanced in the presence of CP (40µg/ml). The most stimulatory neurite outgrowth effects were appeared after 48hrs incubation of neurons with CP (40µg/ml). These data confirm that CP extract could promote invitro hippocampal neurite outgrowth in a dose-dependent manner. Our results indicate that CP can be used as a healthy dietary supplement for the cognitive functions of the brain.

Biosurfactants production potential of native strains of Bacillus cereus and their antimicrobial, cytotoxic and antioxidant activities

2022-12-15T10:38:29Z

Title: Biosurfactants production potential of native strains of Bacillus cereus and their antimicrobial, cytotoxic and antioxidant activities Authors: Madiha Basit, Madiha Basit; Muhammad Hidayat Rasool, Muhammad Hidayat Rasool; Naqvi, Syed Ali Raza; Muhammad Waseem, Muhammad Waseem; Bilal Aslam, Bilal Aslam Abstract: Present study was designed to evaluate the biosurfactant production potential by native strains of Bacillus cereus as well as determine their antimicrobial and antioxidant activities. The strains isolated from garden soil were characterized as B. cereus MMIC 1, MMIC 2 and MMIC 3. Biosurfactants were extracted as grey white precipitates. Optimum conditions for biosurfactant production were 37°C, the 7th day of incubation, 0.5% NaCl, pH 7.0. Moreover, corn steep liquor was the best carbon source. Biuret test, Thin Layer Chromatography (TLC), agar double diffusion and Fourier Transform Infrared Spectroscopy (FTIR) characterized the biosurfactants as cationic lipopeptides. Biosurfactants exhibited significant antibacterial and antifungal activity against S. aureus, E. coli, P. aeruginosa, K. pneumoniae, A. niger and C. albicans at 30 mg/ml. Moreover, they also possessed antiviral activity against NDV at 10 mg/ml. Cytotoxicity assay in BHK-21 cell lines revealed 63% cell survival at 10 mg/ml of biosurfactants and thus considered as safe. They also showed very good antioxidant activity by ferric-reducing activity and DPPH scavenging activity at 2 mg/ml. Consequently, the study offers an insight for the exploration of new bioactive molecules from the soil. It was concluded that lipopeptide biosurfactants produced from native strains of B. cereus may be recommended as safe antimicrobial, emulsifier and antioxidant agent.

Zein based magnesium oxide nanoparticles: Assessment of antimicrobial activity for dental implications

2022-12-15T10:37:27Z

Title: Zein based magnesium oxide nanoparticles: Assessment of antimicrobial activity for dental implications Authors: Naguib, Ghada H; Khaled M Hosny, Khaled M Hosny; Ali H. Hassan, Ali H. Hassan; Faten Al Hazmi, Faten Al Hazmi; yman Al Dharrab, yman Al Dharrab; Hala M Alkhalid, Hala M Alkhalid; Mohamed T Hamed, Mohamed T Hamed; Abeer M Alnowaiser, Abeer M Alnowaiser; Pashley, David H Abstract: MgO nanoparticles have been recently discovered as an antibacterial, however, they limited by property degradation due to agglomeration. The addition of a coating agent, such as a zein polymer, is effective in preventing agglomeration without affecting nanosized properties. The aim of this study was to assess the antimicrobial property of MgO nanoparticles when coated with a zein polymer against several oral bacteria and fungi. This was done by utilizing various assessment techniques. The ultimate aim is to use these nanoparticles in dental preparations. The antimicrobial activity of zein-coated MgO nanoparticles at different concentrations of 0.5, 1 and 2% were tested against four different microorganisms: Staphylococcus aureus, Streptococcus mutans and Enterococcus faecalis (gram positive bacteria), and Candida albicans (as oral fungus). Two different techniques were utilized: the Kirby-Bauer test, and a modified direct contact test. The results indicated that the antibacterial effect of 1% or 2% zein-coated MgO nanowires were statistically significant (p<0.05) against the four organisms studied: S. mutans, S. aureus, E. faecalis and C. albicans. Zein-coated MgO nanoparticles are a new human friendly and potent antimicrobial agent that can be incorporated in the formulation of a variety of new dental materials and products that should provide improvements in dental care and oral health.