http://localhost:80/xmlui/handle/123456789/14331 2026-04-04T17:09:24Z 2026-04-04T17:09:24Z Asif Nadeem Muhammad Rizwan Ashraf Maryam Javed Tanveer Hussain Muhammad Salman Tariq Masroor Ellahi Babar http://localhost:80/xmlui/handle/123456789/15297 2022-12-19T10:34:17Z 2018-10-04T00:00:00Z

Title: REVIEW MicroRNAs: A new paradigm towards mechanistic insight of diseases Authors: Asif Nadeem; Muhammad Rizwan Ashraf; Maryam Javed; Tanveer Hussain; Muhammad Salman Tariq; Masroor Ellahi Babar Abstract: : In 1993 miRNAs were discovered during a research on Caenorhabditis elegans conducted by Victor Ambros and Gary Ruvkun. The gene lin-4 that played important role in development in C. elgans was observed not encoding any protein but a very small RNA molecule of just 22 nucleotides. Main objective of this review is to highlight the significance of miRNAs in regulating the expression of many genes, which are either directly or indirectly involved in many diseases. One of the major causes of illness and death in developed countries of the world is cardiovascular disease. Some of the miRNAs have certain role to play in heart that are not specified for heart. So miRNAs have been found to be in other tissues like fibroblasts, endothelial cells and smooth muscle cells that are part of physiological study of cardiovascular system. Adult heart has limited capacity of regeneration therefore lost cardiomyocytes due to myocardial ischemia or infarction can result in low performance of heart. miRNAs have been shown to play a role in apoptotic regulation of cardiomyocytes in vivo. Many studies have shown that miR146a and 155 are up regulated in peripheral blood mononuclear cells, synovial fibroblasts, synovial fluid and Th-17 cells from rheumatoid arthritis patients as compared to healthy persons. Several types of miRNAs are playing important roles in type 1 diabetes mellitus including miR-375 and miR-375 with intolerance to glucose and decreased beta cells account due to impaired proliferation. Up regulation of miR-125a in WAT of type 2 Diabetes mellitus have been observed. miRNAs have proved to be the important regulators of cytokines and growth factor expression. Thus, suggested as a good biomarker and target of therapy. miRNA profiling techniques have revealed the role of miRNAs in Multiple sclerosis.

2018-10-04T00:00:00Z Bhatti, Muhammad Umar Haseeb Amir Riaz Bushra Tabassum Nida Toufiq Khan, Anwar Muhammad Tariq Iqra Yousaf Imtiaz Ahmad Sajid Mariam Shahid Maryam Zameer http://localhost:80/xmlui/handle/123456789/15296 2022-12-19T10:33:28Z 2018-10-03T00:00:00Z

Title: MINI REVIEW Epigenetics: Quest for no-escape to HIV, a persistent pathogen Authors: Bhatti, Muhammad Umar; Haseeb Amir Riaz; Bushra Tabassum; Nida Toufiq; Khan, Anwar; Muhammad Tariq; Iqra Yousaf; Imtiaz Ahmad Sajid; Mariam Shahid; Maryam Zameer Abstract: Acquired Immune Deficiency Syndrome (AIDS) is a disease infection mix, which is primarily because of 'deficient' immune system. Human Immune-deficiency Virus (HIV) makes the immune system susceptible to many infections by infiltrating it. Many researchers believe that HIV is a mutated form of Simian Immune-deficiency Virus (SIV). After being clinically discovered in 1981 in America, it is said to have caused 36 million deaths. Treatment of AIDS has been a 'burning ' issue ever since its discovery. There is no cure for AIDS! Although, Recombinant Transcriptase Inhibitors (RTis) are being considered a major treatment against HIV that can not only lessen the effect of HIV but also can prolong the life of HIV positive patients. More recent advancement includes 'transplantation of transgenic stem cells' in HIV positive patients. As latency of HIV provirus in host genome is the preeminent weapon of this virus against RTis that compel it to hide from host immune system and a persistent pathogen thereof. Thus, epigenetic activation of latent provirus pool by methyl inhibitors along with nontoxic chemical drugs seems to be a more promising treatment to avoid the burden of lifelong RTI.

2018-10-03T00:00:00Z Nazima Batool Muhammad Arshad Fayyaz-ul-Hassan Noshin Ilyas Armghan Shahzad http://localhost:80/xmlui/handle/123456789/15295 2022-12-19T10:32:44Z 2018-10-02T00:00:00Z

Title: REPORT Physicochemical and Antimicrobial properties of canola (Brassica napus L.) seed oil Authors: Nazima Batool; Muhammad Arshad; Fayyaz-ul-Hassan; Noshin Ilyas; Armghan Shahzad Abstract: Canola oil has been used in the Pakistan for the treatment of various diseases and skin infections. Oil was extracted with n-hexane from the seeds of canola (Brassica napus L.) and was evaluated for free fatty acid value. Four microorganisms namely; Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas originals, and Klebsiella pneumonia, has known to cause some infections treatable with these oils were investigated. The results showed that all oil shown inhibitory effects against Klebsiella pneumoniae, Staphylococcus epidermidis, and Pseudomonas originals but no inhibitory effects was found against Staphylococcus aureus.

2018-10-02T00:00:00Z Luo, Yi Qin, Hongxia Zhao, Lin Jin, Long Jiang, Anan Li, Xuewei Li, Mingzhou Wang, Xun http://localhost:80/xmlui/handle/123456789/15293 2022-12-19T10:30:01Z 2018-10-01T00:00:00Z

Title: REPORT Ouabain inhibits RAW264.7 cells proliferation and induces apoptosis via Bcl-2 and bax expression Authors: Luo, Yi; Qin, Hongxia; Zhao, Lin; Jin, Long; Jiang, Anan; Li, Xuewei; Li, Mingzhou; Wang, Xun Abstract: Ouabain is used in the clinical treatment of the cardiovascular diseases and it can be extensively investigated due to its promising pharmacological, pathological, and physiological properties. The present study is aimed to assess the growth and apoptotic potential of ouabain in RAW 264.7 cells. Varying concentrations of ouabain were applied to cultured RAW 264.7 cells for different time. At 0.01 to 1.00mM doses, ouabain inhibited the proliferation and presented cytotoxicity in both dose- and time-dependent manner. The compound also increased apoptosis after 24 and 48 hours of exposing to 1.00mM dose. Furthermore, the expression of Bcl-2 mRNA significantly decreased following ouabain treatment while Bax mRNA expression increased significantly. In brief, ouabain inhibits cell growth, exerts cytotoxicity and induces apoptosis in RAW 264.7 cells by down-regulating the Bcl-2/Bax ratio at mRNA level.

2018-10-01T00:00:00Z