http://localhost:80/xmlui/handle/123456789/14448 2026-04-11T16:35:16Z 2026-04-11T16:35:16Z Ghufran, Hafiz Riaz, Sehar Mahmood, Nasir Yaqoob, Muhammad Shahid, Saman Bhinder, Munir Ahmad Anwar, Saadia Faizan, Mahwish http://localhost:80/xmlui/handle/123456789/14668 2022-12-05T09:49:18Z 2019-09-21T00:00:00Z

Title: Polymorphism of Interleukin-10 (IL-10, -1082 G/A) and Interleukin28B (IL-28B, C/T) in pediatric Acute Lymphoblastic Leukemia (ALL) Authors: Ghufran, Hafiz; Riaz, Sehar; Mahmood, Nasir; Yaqoob, Muhammad; Shahid, Saman; Bhinder, Munir Ahmad; Anwar, Saadia; Faizan, Mahwish Abstract: We conducted genotypic analyses of interleukin-10 (IL-10) (-1082 G/A; GG, GA, AA) and interleukin-28B (CC, CT, TT) genes polymorphisms in acute lymphoblastic leukemia (ALL) pediatric patients in descriptive study to evaluate the prevalence of these mutations. In amplification refractory mutation system-PCR (ARMS-PCR), one reaction was carried out for each patient’s DNA sample. For IL-28B gene, two forward and two reverse primers specific for Callele and T-allele were used separately. For human IL-10 gene, two different forward primers specific for A and G alleles were used in combination with common reverse primer. IL-10 gene promoter showed highest frequency (n=29, 58%) of heterozygous (GA) allele, while genotypic analysis of IL-28B gene showed highest frequency (n=28, 56%) of homozygous (CC) allele. The IL-10 (AA) genotype related to its protein’s less production in body which may be associated with the least survival of ALL’s patients, while IL-28b (CT and TT) genotypes may be associated with less IFNλ3 levels and less life expectancy.

2019-09-21T00:00:00Z Hanif, Muhammad Mehmood, Malik Hassan Ishrat, Ghazala Virji, Safna Naozer Malik, Abdul Ahmed, Mansoor Gilani, Anwarul-Hassan http://localhost:80/xmlui/handle/123456789/14667 2022-12-05T09:42:57Z 2019-09-20T00:00:00Z

Title: Pharmacological basis for the medicinal use of Alcea rosea in airways disorders and chemical characterization of its fixed oils through GCMS Authors: Hanif, Muhammad; Mehmood, Malik Hassan; Ishrat, Ghazala; Virji, Safna Naozer; Malik, Abdul; Ahmed, Mansoor; Gilani, Anwarul-Hassan Abstract: Alcea rosea L. also known as Althea rosea belongs to the Malvaceae family. This medicinal herb, traditionally used to treat several conditions including airway disorders like asthma and chronic bronchitis. This study evaluated the bronchodilatory effects and possible mechanism of A. rosea on guinea-pig tracheal tissues. Moreover lipophilic profiling of A. rosea has been carried out by using Gas-Chromatography-Mass-Spectrometry. A total of 19 compounds have been identified from the plant, n-hexane fraction. These compounds have been further confirmed from their Van den Dool and Kratz (I) Indices. Major class of metabolite identified from the plant includes fatty acid, saturated and unsaturated fatty acid esters. Hydrocarbons have also been detected from the n-hexane fraction. These fatty acid esters have not been reported previously by GC-MS and were identified first time from the flowers of Alcea rosea. In-vitro experiments were performed on guinea-pig tracheal tissues, mounted in Kreb’s solution at 37°C and bubbled with carbogen. In isolated guinea-pig trachea, A. rosea inhibited carbamylcholine and K+ (80 mM)-induced contractions, potentiated isoprenaline concentration-response curves (CRCs) and suppressed Ca2+ CRCs. These results suggest that A. rosea cause bronchodilation through dual inhibition of phosphodiesterase enzyme and Ca2+ influx, which substantiate its potential in airways disorders.

2019-09-20T00:00:00Z Mirza, Rabia Azeem, Muhammed Qaisar, Uzma http://localhost:80/xmlui/handle/123456789/14666 2022-12-05T09:37:12Z 2019-09-19T00:00:00Z

Title: Influence of Peganum harmala peptides on the transcriptional activity of biofilm related genes in sensitive and resistant strains of Pseudomonas aeruginosa and Staphylococcus aureus Authors: Mirza, Rabia; Azeem, Muhammed; Qaisar, Uzma Abstract: Microbial biofilms have gathered interest in recent years as they have become the major cause of nosocomial infections. The abuse and misuse of antibiotics have created a selective pressure that results in widespread formation of resistant bacterial strains and a need to devise novel plant based antimicrobials. In this study, antimicrobial peptides were isolated from Peganum harmala and their effect was examined on biofilm related colonization genes of Pseudomonas aeruginosa and Staphylococcus aureus isolated from burn and surgical wounds. Results showed that in P. aeruginosa isolated from burn wound, the expression of flagellar gene (flgK), pilin gene (pilA) and fimbriae gene (cupA1) was significantly down-regulated indicating that Peganum harmala antimicrobial peptides (PhAMP) damage locomotors of planktonic cells by affecting the gene expression while in resistant biofilm cells, the expression of flgK, cupA1 and polysaccharide synthesis gene (pslA) was enhanced in the presence of PhAMP. In P. aeruginosa isolated from surgical wounds which was more sensitive; the expression of flgK, pilA, cupA1 and pslA was significantly down-regulated in biofilms and planktonic cells in the presence of PhAMP thus disrupting locomotors of planktonic as well as biofilm cells. In S. aureus isolated from burn wounds; the expression of capsular polysaccharide synthesis gene (CPS5) and inter cellular adhesion gene (icaA) was significantly up-regulated in biofilms as well as in planktonic cells in response to PhAMP stress showing resistance mechanism. Thus these genes can be used as efficient resistance markers for bacterial pathogens against antimicrobial agents.

2019-09-19T00:00:00Z Zaheer, Jawad Saqib, Qazi Najam-Us- Hashmi, Asif Mehmmod Mukhtiar, Muhammad Zafar, Sadia Riaz, Muhammad Rasool, Ghulam Akram, Muhammad Shah, Syed Muhammad Ali Rahat, Saher Khan, Faid Said http://localhost:80/xmlui/handle/123456789/14665 2022-12-05T09:34:17Z 2019-09-18T00:00:00Z

Title: Ex-vivo abortifacient activity of Androsace foliosa n-hexane leaves extract on isolated rabbit uterus Authors: Zaheer, Jawad; Saqib, Qazi Najam-Us-; Hashmi, Asif Mehmmod; Mukhtiar, Muhammad; Zafar, Sadia; Riaz, Muhammad; Rasool, Ghulam; Akram, Muhammad; Shah, Syed Muhammad Ali; Rahat, Saher; Khan, Faid Said Abstract: Androsace foliosa is a medicinal herb utilized in different areas of Pakistan for abortifacient, diabetic and liver complications. In the current research, the possible action of the n-hexane leaves extract of the Androsace foliosa on isolated rabbit uterus was examined. Abortifacient activity was examined in the existence of standard antagonist e.g. atropine and salbutamol and a uterine tonic like oxytocin. The isolated rabbit uterus is initially treated with 1mg/kg stilboesterol for 1 complete day. The consequence of oxytocin as uterine contraction agonist was observed. Additionally, antagonists e.g. salbutamol (2µg) and atropine (1-2mg) on the uterine contractile action of the extract were also examined. The A. foliosa n-hexane leaves extract fashion dose correlated amplification in the force of uterine contraction comparable to oxytocin. The drug oxytocin was pragmatic to amplify the uterine contractile action of the extract. Meanwhile pre-treating the tissue with either atropine or salbutamol earlier than administrating the extract indicates the inhibitory action of the drugs on the action of the extract.

2019-09-18T00:00:00Z