http://localhost:80/xmlui/handle/123456789/14697 2026-04-23T15:33:52Z 2026-04-23T15:33:52Z MUJAHID HUSSAIN IKRAM-UL-HAQ SIKANDER ALI M.A. QADEER http://localhost:80/xmlui/handle/123456789/15351 2022-12-20T04:48:02Z 2012-07-20T00:00:00Z

Title: BIOSYNTHESIS OF L-PHENYLACETYLCARBINOL FROM LOCALLY ISOLATED YEASTS Authors: MUJAHID HUSSAIN; IKRAM-UL-HAQ; SIKANDER ALI; M.A. QADEER Abstract: In the present study, 250 yeast strains were isolated from samples of different natural sources as cane-molasses, decaying vegetables and bagasse using glucose enriched medium. Among these, 106 strains showed no growth in acetaldehyde (1 g/l) supplemented yeast extract-peptone dextrose plates during qualitative screening. In the course of quantitative screening, 64 acetaldehyde tolerants gave almost negligible L-PAC production (≤0.5 g/l) using glucose-peptone medium in shake flasks. A comparatively better L-PAC production was observed with the rest of strains. The isolate Saccharomyces cerevisiae GCU-36 exhibited higher L-PAC production (2.58 g/l). However, lower sugar consumption and subsequent biomass formation was noted. Therefore, yeast GCU-36 was selected as a hyperproducer of L-PAC in batch culture.

2012-07-20T00:00:00Z MUHAMMAD ANIS M. JAVED ZAKI SHAHNAZ DAWAR http://localhost:80/xmlui/handle/123456789/15350 2022-12-20T04:47:37Z 2012-07-19T00:00:00Z

Title: DEVELOPMENT OF A Na-ALGINATE-BASED BIOFORMULATION AND ITS USE IN THE MANAGEMENT OF CHARCOAL ROT OF SUNFLOWER (HELIANTHUS ANNUUS L.) Authors: MUHAMMAD ANIS; M. JAVED ZAKI; SHAHNAZ DAWAR Abstract: The treatment of sunflower seeds with Na-alginate in combination with Ca-carbonate and carboxymethyl cellulose (CMC) showed maximum increase in plant length and weight followed by seed coated with Na-alginate in combination with Ca-carbonate and gum arabic. Maximum control of Macrophomina phaseolina infection was recorded when seeds were coated with Na-alginate in combination with Ca-carbonate and gum arabic followed by Na-alginate in combination with Cacarbonate and CMC. In another experiment when sunflower seeds were coated with Na-alginate at 1, 2 and 4% w/w with or without Trichoderma viride, T. resei, significant increase in germination was recorded when seeds were coated with Trichoderma species using Na alginate at different concentrations as a sticker. Plant length and weight was significantly higher in treated seeds as compared to non treated control. Seeds coated with T. viride using 2% Na-alginate as a sticker showed maximum increase in plant length and weight followed by seed coated with T. viride in combination with 1% Naalginate. Maximum vigor index were observed in sunflower seeds treated with T. viridi using 2% Na-alginate.

2012-07-19T00:00:00Z KHAN, M. YAKOUB M. UMAR DAHOT S.HABIB AHMED NAQVI http://localhost:80/xmlui/handle/123456789/15349 2022-12-20T04:47:04Z 2012-07-18T00:00:00Z

Title: AMINO ACIDS COMPOSITION OF MYCELIAL PROTEIN OF ENICILLIUM EXPANSUM GROWN IN ACID TREATED RICE HUSK MINERAL MEDIUM Authors: KHAN, M. YAKOUB; M. UMAR DAHOT; S.HABIB AHMED NAQVI Abstract: The aim of the present study was to analyze the amino acids composition of single cell protein of Penicillium expansum. Mycelial biomass was produced when fungus was grown in 0.6N H2SO4 pretreated rice husk mineral medium incorporated with 0.5% and 1% of nitrogen sources like potassium nitrate, sodium nitrate, ammonium nitrate, peptone, yeast extract, urea, corn steep liquor and ammonium sulphate. It was observed that the growth rate of Penicillium expansum increased with 0.5% sodium nitrate produces 1.390 ± 0.084g/l of mycelial biomass. In the subsequent experiment, fermentation medium was supplemented with 0.5% and 1.0% different sugars (sucrose, glucose, fructose, maltose, galactose, lactose, carboxymethylcellulose, starch, mannose, and molasses) at pH 6.0 for 240 hours at 35 ± 2°C in a fermenter. The highest amount of mycelial biomass (5.107 ± 0.169g/l) was obtained with 1% sucrose and followed by 4.953 ± 0.17g/l, 4.808 ± 0.14g/l and 4.844 ± 0.10g/l mycelial biomass using glucose, maltose and galactose, respectively. The mycelial biomass of Penicillium expansum contains essential and non essential amino acids like phospho-serine, serine, valine, aspartic acid, threonine, glutamic acid, glycine, isoleucine, leucine, phenylalanine, alo-lysine, halo-lysine, lysine and arginine. The glutamic acid (3355.0 ± 19.798 μmol/g mycelia) and proline (785.0 ± 9.899 μmol/g mycelia) were found in higher concentration than other amino acids produced by Penicillium expansum grown on rice husk supplemented with lactose.

2012-07-18T00:00:00Z ABEER HASHEM E.F. ABD-ALLAH HEND A. ALWATHNANI http://localhost:80/xmlui/handle/123456789/15348 2022-12-20T04:46:23Z 2012-07-17T00:00:00Z

Title: EFFECT OF PROPOLIS ON GROWTH, AFLATOXINS PRODUCTION AND LIPID METABOLISM IN ASPERGILLUS PARASITICUS SPEAR Authors: ABEER HASHEM; E.F. ABD-ALLAH; HEND A. ALWATHNANI Abstract: The mechanism of antifungal activities of propolis (Bee glue) was investigated for the growth, aflatoxins production, and lipids (total lipids, neutral lipids, phospholipids, and fatty acids) metabolism of Aspergillus parasiticus. The results of the present study indicated that propolis caused significant decrease in conidial production and conidial germination as well as mycelial growth (both radial and dry weight) of A. parasiticus. It was also found that aflatoxins production by A. parasiticus decreased significantly with 0.2 and 0.4 (g/100 ml) concentrations of propolis, however, 0.6 (g/100 ml) concentration caused complete inhibition of all aflatoxins production. The biochemical investigation of cellular total lipids, neutral lipids, and phospholipids of A. parasiticus suggested clear catabolic repression of lipids metabolism by propolis. Gas chromatographic analysis of cellular fatty acids indicated that propolis enhanced accumulation of saturated fatty acids suggesting resistance mechanism of fungal membrane via decreasing its fluidity and elasticity.

2012-07-17T00:00:00Z