http://localhost:80/xmlui/handle/123456789/12229 2026-04-24T01:10:46Z http://localhost:80/xmlui/handle/123456789/12235 Title: Production and Characterization of Antisera Against Steroids in Rabbits Authors: MAHMOOD, ARSHAD; BAIG, SHAHID MAHMOOD; KHAN, M. NASIM; MALIK, SALMAN A.; KHANUM, AZRA Abstract: Antibodies against testosterone, progesterone and estradiol were raised by immunization of rabbits with testosterone-3-(o-carboxyrnethyl) oxime-bovine serum albumin (testosterone-3-BSA), progesterone-11-(o carboxymethyl) oxime-bovine serum albumin (progesterone 11-BSA), and estradiol-6-(0-carboxymethyl) oxime-bovine serum albumin (estradiol-6-BSA). The antisera have shown a high titer of 48-54% binding at the final dilution of 1:24,000. These antisera, therefore, have shown high affinity. The affinity: constant (Ka) calculated for testosterone, progesterone and estradiol antisera were 6.5x109 1/mol, 3.2x109 1/mol, and 0.29x109 1/mol, respectively. The specificity and cross-reactivity results have shown a high degree of specificity toward their respective homologous steroids. The mean percent recovery of unlabelled hormones added to samples was 105±10.63 for testosterone, 92.75±2.77 for progesterone and 107±5.38 for estradiol antisera. The sensitivity of testosterone, progesterone and estradiol standard of testosterone, progesterone and estradiol standard curves was 10,23 and picograms per 200 1, respectively, when calculated at 95% confidence limit. 1991-07-01T00:00:00Z http://localhost:80/xmlui/handle/123456789/12234 Title: Studies on the In Vitro and In Vivo Release of Hormonal Steroids Bound to a Polymer Base Authors: KHAN, S.; ANJUM, S.; BAIG, S.M.; KHANUM, A.; HAIDER, M.Z.; QAZI, M.H. Abstract: Poly (ethylene) glycol (PEG) based hydrogels crosslinked with 1,1,1, tris (hydroxy methyl) ethane, in phase separated hydrogcls and 1,2,6, hexane triol in non-phase ·separated hydrogels were used to study the characteristics of release of hormonal steroids testosterone, progesterone and estradiol. A rapid in vitro release of steroids was observed with lM PEG-6000 non-phase separated gel. A dramatic slowing down in the in vitro release characteristics was observed in the case of phase-separated gels and with increasing crosslinking. The time for 50% release (t0.s) for the steroids was of the order of 9.Sh, 10.Sh and 12h for 1.5M, 2M and 3M PEG-6000 hydrogels, respectively. The 3M PEG-6000 phase separated gels retained the same characteristics of release in vivo since only 2.0% of 3H-progesterone was released by 72h. The data demonstrates that PEG based hydrogels with appropriate crosslinking can be used effectively for sustained release of steroids from the site of implantation. 1991-07-01T00:00:00Z http://localhost:80/xmlui/handle/123456789/12233 Title: Serum LH, FSH and Estradiol Levels in Women Using Injectable Contraceptive (Norigest) Over Long Periods of Time Authors: Ahmed, M. Maqbool; Todehdehghan, F; Baig, Shahid Abstract: Blood glucose, serum bilirubin, serum enzymes, i.e. SAP, SGPT and SGOT, sodium and potassium, LH, FSH and estradiol level were determined in 93 women (aged 25-45). These subjects were grouped according to the time of receiving intragluteal injections (200 mg NETEN 8-weeks intervals). The groups of women receiving injections for 19-24 months showed a significant decrease (P < 0.01) in LH, FSH and estradiol levels. The pregnancy rate was 2%. Levels of LH, FSH and estradiol were elevated in women receiving injections for 25-38 months. The pregnancy rate was 3%. 17,e values were not significantly different when compared with controls. A significant increase in serum bilirubin, SAP, SGPT, SGOT, sodium and potassium levels were recorded in all treated groups. 17,e control and treated groups did not show any significant difference with respect to blood glucose levels. 1989-07-01T00:00:00Z http://localhost:80/xmlui/handle/123456789/12232 Title: Circulating Levels of 17--Estradiol, Testosterone and Progesterone in Postmenopausal Breast Cancer Patients Receiving Anticancer Chemotherapy Authors: BAIG, S.M.; ANJUM, S.; KHAN, S.; KHANUM, A.; QAZI, M.H.; HAIDER, M.Z. Abstract: Plasma levels of steroid hormones (17/1-estradiol, testosterone and progesterone were determined in postmenopausal women suffering from breast cancer, cancers other than breast and normal healthy subjects. The levels of all steroids were considerably higher in breast cancer patients than the other two groups. During the five month sampling period, estradiol levels decreased in breast cancer patients after CMF (cyclophosphamide, methotrexate, 5-flourouracil) therapy, but there was no significant change in the concentration of testosterone and progesterone. In the patients suffering from cancers other than breast and healthy controls, the levels of all the three steroids remained steady throughout the sampling period. Knowledge of the hormonal status of postmenopausal women facilitates the choice and timing of chemotherapy for controlling the disease. 1991-09-01T00:00:00Z