http://localhost:80/xmlui/handle/123456789/12723 2026-04-23T22:17:51Z http://localhost:80/xmlui/handle/123456789/13897 Title: CYP2D6*4 null allele frequency in sixteen Pakistani ethnic groups Authors: Anwar Ullah; Riaz, Sana; Siddiqi, Saima; Mazhar, Kehkashan; Mansoor, Atika Abstract: : CYP2D6 belongs to a family of Cytochrome P450 and is involved in metabolism of a number of commonly prescribed drugs. This study was designed to identify *4 allelic frequency of CYP2D6 in Pakistani population. The ethno-geographic variations in the CYP2D6 alleles are responsible for varied expression of this enzyme and thus influence the metabolic rate and efficacy of prescribed drugs. In total, 976 volunteers belonging to 16 different ethnic groups of Pakistan were screened for CYP2D6*4 polymorphism. The *4 allele was detected in all the ethnic groups with varied frequency ranging from 3.73%-13.64% and an overall average of 7.22% in different ethnic groups of the population. Maximum frequency was detected in northern Pakistani population including Meo (13.64%), Punjabi (11.96%) and Pathan (10.42%). Low frequency (<4%) of*4 polymorphism was observed in Kalash and Makrani groups, whereas an intermediate frequency (5-9%) was observed in all the other ethnic groups. The data indicates that despite ethnic diversity poor metabolizers in Pakistani population are expected to carry CYP2D6*4 allele at a relatively higher frequency than most other Asian populations. (Word count = 186) 2020-03-16T00:00:00Z http://localhost:80/xmlui/handle/123456789/13896 Title: Molecular docking and in-silico study of natural antagonists of ERalpha receptor: Potential candidates against breast cancer Authors: Ahmed, Bilal; Qadir, Muhammad Imran; Hafiza Noor-ul-Ain Abstract: Breast cancer is affecting the women in both developing and non-developing countries in a dangerous ratio. Estrogen receptor alpha (ER-α) controls a number of physiological processes. Its over expression is seen in breast cancer. Estrogen positive breast cancer can be treated by hormone therapy, that’s why we focused on anti-estrogen natural molecules. A library of 5022 phytochemicals was searched, and selected top molecules based on their drug scoring. Fulvestrant was used as the reference drug. 4 molecules showed more drug scoring than the standard and these include Kushenol K, Flavobin, Kushenol N and CHEMBL66996 in descending order. All these molecules were further assessed for their other biological activities and found better than the standard. Therefore, these compounds may be used effectively as antagonists of ER-alpha receptor and as potential candidates against breast cancer. 2020-03-16T00:00:00Z http://localhost:80/xmlui/handle/123456789/13895 Title: Therapeutic effects of Rhamnus alaternus on the nephroangiosclerosis in wistar rats Authors: Halzoune, Hanane; Saiah, Wassila; Tabani, Khadija; Lahfa, Farid; Koceir, Elhadj Ahmed; Omari, Naima Abstract: The buckthorn “Rhamnus alaternus” is a plant used in traditional medicine especially in the treatment of certain diseases such as diabetes, cardiovascular disease and dyslipidemia. The aim of our study was to analysed and evaluate the effects of buckthorn on nephroangiosclerosis Wistar rats. Thirty male Wistar rats, adult weighing between 120g and 250g were distributed as follows: Five control animals received a standard laboratory diet, thirty four experimental rats received the standard laboratory diet supplemented with palm oil and 4% of NaCl (High Sold Fat Diet). After six month of this diet the HSFD group was subdivided into rats treated for 45 days with aqueous extract of buckthorn or animals HSFD only. Plasma metabolites and endothelin-1 concentration were measured by standard methods, section of kidney were stained by Heindenhain-azan and periodic acid shiff. The examination of renal parenchyma of HSFD rats showed a prominent structural changes such as, obstruction of vascular lumen, ischemia of the glomerular and tubulo-interstitial fibrosis the biochemical and hormonal parameters were significantly improved in the HSFD rats treated with decoction of buckthorn. Moreover, the morphogical changes of the renal parenchyma were attenuated in rats HSFD decoction. The buckthorn attenuates renal parenchymal lesions by aggregating at different levels of rats maintained on HSFD. 2020-03-16T00:00:00Z http://localhost:80/xmlui/handle/123456789/13894 Title: In-silico determination of pKa and logp values of some isoniazid synthetic analogues using Marvin software Authors: Naeem, Sabahat; Akhtar, Shamim; Zafar, Shaista; Iqbal, Sadia Abstract: : Among the physicochemical properties, pKa and LogP values help us in studying drug parameters like ADME and could be predicted to some extent. With this view, here we wish to predict these two properties of our previously synthesized biologically active derivatives of isoniazid using on-line available program Marvin, a Java-based chemical software application frequently used for chemical modeling. According to Marvin, pKa values predicted 99.99% unionized states of INH and some derivatives at physiological pH 7.4. Marvin calculated LogP values estimated good oral absorption for all the synthesized compounds. Therefore it can be said that the findings of the study emerged in an ideal region that permits the formulation of these derivatives. Since this was just a theoretical study, it demands more experimentation to determine accurate situation. 2020-03-16T00:00:00Z