http://localhost:80/xmlui/handle/123456789/14451 2026-03-03T03:57:59Z http://localhost:80/xmlui/handle/123456789/14611 Title: Enhanced dissolution rate of Ketoprofen by fabricating into smart nanocrystals Authors: Khan, Jahangir; Bashir, Sajid; Khan, Muhammad Asif; Ghaffar, Rukhsana; Naz, Attiqa; Khan, Wali; Ahmad, Shujat; Ullah, Abid; Ali, Faryal Liaqat; Isreb, Mohammad Abstract: The low bioavailability of Ketoprofen is associated with its hydrophobic nature that can be solved by nanonization. For this purpose, a polymeric solution with drug concentration of 3.5% w/w was formulated. The produced solution was milled for 60 minutes in DENA® mill which contains 0.2µm yttrium reinforced zirconium beads. The Physicochemical properties, characterization including stability studies of the prepared nanoparticles were carried out using pharmacopeial techniques of zeta potential, PXRD, DSC, SEM and TEM. Results suggest that stable crystalline nanocrystals with a size of 169±1.98nm with PDI of 0.194±0.04 and zeta potential of -22.0±2.25mV were produced. Moreover, enhances in-vitro release rate of 78.6% for the processed Ketoprofen was achieved in first 5 min as compared to raw form and marketed drug which released only 22.9% and 33.1% of drug respectively. The 60 days stability studies at 4oC & 25oC revealed that polymers PVP-K30-HPMC-6cps-SDS were effective in stabilizing the nanocrystals. Comparatively stable ketoprofen nanocrystals were successfully produced by DENA® mill with marked enhanced dissolution rate. It proved a useful for commercialization technique due to high drug concentration and retention of distinct characteristics at large scale. 2019-11-19T00:00:00Z http://localhost:80/xmlui/handle/123456789/14609 Title: Ex-vivo antihypertensive and calcium channel blocking activity of Androsace foliosa n-hexane leaves fraction on isolated rabbit aorta Authors: Zaheer, Jawad; Kausar, Abida; Kanwa, Hina; Akhtar, Noreen; Ilyas, Aisha; Nazlli, Zil-I-Huma; Riaz, Ayesha; Shah, Syed Muhammad Ali; Akram, Muhammad; Sarwar, Muhammad Usman; Khan, Abdul Hamid; Khan, Fahad Said; Asad, Farhan; Anwar, Hina; Anwar, Madeeha Abstract: Hypertension is persistent elevation in blood pressure for 3-4 weeks. Estimated global prevalence of hypertension suggested that by the Year 2025 (29%) of adult worldwide are suffering from hypertension (1.56 billion). Hypertension complications are hemorrhage, atherosclerosis, renal artery stenosis, angina pectoris end organ damage, cardiomyopathy, myocardial infarction and retinopathy. Along with other drug class Calcium channel blocker are also used for the treatment of hypertension. In this study the possible action of the n-hexane leaves fraction of the Androsace foliosa on isolated rabbit aorta was examined. Antihypertensive activity was examined in the existence of standard agonist like phenylephrine and antagonist like Verapamil. Phenylephrine (PE 1µM) high K+ was used to steady the tissue materials. Additionally to observe the calcium channel blocking effect the tissues were treated with n-hexane segment of A. foliosa leaves. Aortic tissues were treated 4-5intervals with Ca+2- free preparation earlier to control calcium reaction curve (CRCs). Verapamil is utilized as standard calcium channel inhibitory mediator and is used as an antagonist. The Af. n-hexane leaves fraction completely inhibited the precontractions induced by Phenylephrine (1µM) and K+ (80 mM) precontractions, with EC50 standards of 1.0mM (0.3-1.0mg/mL) and 4.90mM (1-3mg/mL), espectively. Androsace foliosa n-hexane leaves fraction was tested for calcium channel inhibitory effect on isolated rabbit aorta. A. foliosa nhexane leaves segment at the dosage of 1mg/mL block the calcium channel approximately (35±5%). Consequence indicates that A. foliosa n-hexane leaves segment block calcium channel in the similar manner as compared to the standard calcium channel blocker drug (verapamil). 2019-11-18T00:00:00Z http://localhost:80/xmlui/handle/123456789/14607 Title: Antimicrobial profiling and molecular characterization of antibiotic resistant genes of Proteus vulgaris isolated from tertiary care hospital, Islamabad, Pakistan Authors: Bilal, Shahrukh; Anam, Sidra; Mahmood, Tauqeer; Abdullah, Rana Muhammad; Nisar, Sajid; Kalsoom, Furkhanda; Luqman, Muhammad; Anjum, Faisal Rasheed Abstract: Urinary tract infections (UTIs) are among the most common bacterial infections acquired from hospitals and community. Pseudomonas and Proteus species are the common cause of these UTIs. Generally, UTIs are self-limiting but have potential to re-occur. Extensive treatment therapy with antibiotics lead to the development of resistance in uropathogens. The development of antibiotic resistance is leading to the failure of currently available antibiotic based therapies thus making the situation worse. The objective of the present study was to access antimicrobial sensitivity and to characterize antibiotic resistant genes of Proteus vulgaris (P. vulgaris) isolated from patients suffering with UTIs. A total of 150 urine samples were collected and cultured on MacConkey agar medium followed by isolation and identification on blood agar medium. Biochemical characterization of all presumptive Proteus isolates was done using Remel Rap ID one kit. Antibiotic sensitivity for P. vulgaris isolates was performed by disc diffusion method. Presence of blaTEM and qnr antibiotic resistant genes was determined by PCR. The results showed that the overall prevalence of P. vulgaris in clinical samples was 11.3%. It showed maximum resistance (94%) to three antibiotics i.e. ampicillin, tigecycline and chloramphenicol, while least resistance was observed against imipenem (12%). Statistical analysis depicted that imipenem had a significantly larger zone of inhibition (P=.01), while ampicillin had significantly smaller zone of inhibition (P=.0004) followed by chloramphenicol (p-value = 0.002). Imipenem should be considered as an effective antibiotic to treat urinary tract infections associated with P. vulgaris. Both blaTEM and qnr genes were found to be involved in conferring resistance to β-lactam and quinolones antibiotics. 2019-11-17T00:00:00Z http://localhost:80/xmlui/handle/123456789/14606 Title: Compatibility analysis of bergapten with different pharmaceutical excipients used in nanostructured lipid carriers Authors: Arshad, Shumaila; Rehman, Masood-ur; Zulfiqar, Juwairiya; Najam, Saima; Asim, Mulazim Hussain; Abid, Farah Abstract: In pharmaceuticals sciences, Fourier-transform infrared spectroscopy (FTIR) is a very useful technique to measure the compatibility and interaction between ingredients, therefore in the current study, compatibility of bergapten with different excipients was analyzed via FTIR. Nanostructured lipid carriers (NLCs) are the second generation of lipid nanocarriers and very useful for the drug delivery systems. Nanoparticles (NPs) were prepared by a nanotemplate engineering technique and scanning of pure drug, individual ingredients and, physical mixtures of different ingredients was carried out. The characteristic peak of the carboxylic groups of bergapten is shifted from 3088.1 cm-1 to 3399.3 cm-1 due to formulation development and it confirmed that it was properly incorporated into the formulation. Other peaks of the drug were also present in formulation with minor shortening/broadening of peaks. The resulted peaks of IR spectra depicted that the ingredients used in the formulation had no considerable interaction and were found compatible with each other. 2019-11-16T00:00:00Z