http://localhost:80/xmlui/handle/123456789/14472 2026-04-23T14:35:46Z http://localhost:80/xmlui/handle/123456789/15858 Title: QUANTITATIVE CHANGES IN PROTEINS, PIGMENTS AND SENNOSIDES OF CASSIA ANGUSTIFOLIA VAHL TREATED WITH MANCOZEB Authors: UZMA MAJID; MAHMOODUZZAFAR; TARIQ OMAR SIDDIQI; IBRAHIM MOHAMMAD AREF; MUHAMMAD IQBAL Abstract: Seeds of Cassia angustifolia Vahl treated with different concentrations (0%, 0.1%, 0.15%, 0.2%, and 0.25%) of a fungicide, Mancozeb, were sown in field conditions in order to study the effect of the stress caused by this fungicide on pigment concentration and the protein and sennoside contents of the plant. Protein content and pigment concentration increased at 0.10% dose of Mancozeb, but higher doses caused a significant decline in comparison with the control. Application of Mancozeb resulted in a considerable dose-dependent reduction in the contents of sennoside a (nearly 7-32% reduction in young leaves, 14-36% in mature leaves and 8-24% in pods) and sennoside b (about 11-36% reduction in young leaves, 5-38% in mature leaves and 5-30% in pods), thus affecting indirectly the therapeutic property of the plant. 2013-11-19T00:00:00Z http://localhost:80/xmlui/handle/123456789/15857 Title: PHYTOCHEMICAL INVESTIGATIONS OF TAMARIX INDICA WILLD. AND TAMARIX PASSERNIOIDES DEL. ex DESV. LEAVES FROM PAKISTAN Authors: FARAH NAZ; SYEDA QAMARUNNISA; SHINWAR, ZABTA KHAN; ABID AZHAR; SYED IRTIFAQ ALI Abstract: Plant-derived chemicals play an important role in protection against invading microbes that cause many infectious diseases and also found to be beneficial for therapeutics in prevention of many other noninfectious ailments. This research involves the investigation of such bioactive chemicals using leaves of two common Tamarix species. Qualitative and quantitative analysis using four different solvents showed methanol has the best extraction potential followed by acetone, ethanol and chloroform. Tamarix indica was found to contain most of the metabolites in higher quantity as compared to Tamarix passernioides. 2013-11-18T00:00:00Z http://localhost:80/xmlui/handle/123456789/15856 Title: INHERITANCE OF MORPHOLOGICAL CHARACTERS ASSOCIATED WITH PLANT AND DRIED SEEDS IN LENTIL (LENS CULINARIS MEDIK.) Authors: AHMAD BAKHSH; SH. MUHAMMAD IQBAL; NASIR M. CHEEMA Abstract: Inheritance of testa colour, testa pattern, cotyledon color and stem color was studied in crosses of lentil involving parents with brown, grey, green testa color, red and yellow cotyledon color, and with green and colored seedling stem. Analysis of F1 plant populations of crosses between green and colored (pink) stem revealed that colored stem was dominant over green stem. Similarly red cotyledon was dominant over yellow cotyledon. The F2 segregation patterns indicated red cotyledon and colored stem to be monogenic traits. The analysis of F2 and F3 seeds (having F1 and F2 testa, respectively) revealed that spackled testa was dominant to even (without speckles) testa, grey testa was dominant to green testa and brown testa was dominant to both grey and green testa. The F2 segregation ratio from cross involving parents with brown and grey testa was 9 brown: 3 grey: 4 green. The crosses between parents with grey and green testa color segregated to fit a ratio of 3:1 between dominant and recessive color. These segregating patterns revealed involvement of two genes and a recessive inhibitor in the regulation of testa color. 2013-11-18T00:00:00Z http://localhost:80/xmlui/handle/123456789/15855 Title: A PROTEOMIC APPROACH TO IDENTIFY SALT-RESPONSIVE PROTEINS IN RYE Authors: LEE, KI-WON; CHOI, GI JUN; KIM, KI-YONG; JI, HEE CHUNG; PARK, HYUNG SOO; LEE, SANG-HOON Abstract: Salinity stress negatively impacts agricultural yield throughout the world affecting production whether it is for subsistence or economic gain. Despite their great degree of stress tolerance, minor crops have received little attention regarding their response against salinity compared to model plants and major crops. Rye, an economically important minor grain crop, is relatively tolerant to abiotic stresses and grown in marginal lands. Proteome study based on two dimensional gel electrophoresis and subsequent mass spectrometric identification was performed in order to analyze the salt-stress response of rye. Overall changes in the protein complement of leaves after four days exposure to 200 mM NaCl were studied. Seventeen reproducibly resolved, differentially expressed protein spots were identified by MALDI-TOF MS (mass spectrometry). Among them, ten proteins were up-regulated, and seven proteins were down-regulated. Majority of the differentially expressed proteins belonged to the functional category of antioxidant metabolism and photosyntheis. Increased expression of reactive oxygen species-scavenging enzymes eg, catalase, cytosolic, monodehydroascorbate reductase and 2- cys-peroxiredoxin may have important role in salinity-induced ROS metabolism. This is the first proteomic description of salt stress response in rye and the identification of such differentially expressed proteins would provide deeper insight pertaining to the stress physiology of this important crop plant. 2013-11-16T00:00:00Z