http://localhost:80/xmlui/handle/123456789/14493 2026-04-23T09:33:13Z http://localhost:80/xmlui/handle/123456789/14822 Title: BALLIELLA SHAMEELII SP. NOV. (CERAMIALES, RHODOPHYCOTA) IN THE COASTAL WATERS OF NORTHERN ARABIAN SEA Authors: SHAHNAZ, LAILA Abstract: The feather-like thalli of the red alga; Balliella shameelii Shahnaz sp. nov. were collected as drift material from the seashore waters at Buleji, the coastal area near Karachi (Pakistan) during February and March, 2012. It was investigated in detail morphologically and cytologically including reproduction. This is the first report of occurrence of the genus Balliella Itono et T. Tanaka in the coastal waters of Pakistan. The new species is characterized by the development of alternate laterals on every 2nd or 3rd axial cell 2015-01-21T00:00:00Z http://localhost:80/xmlui/handle/123456789/14820 Title: ANTIFUNGAL AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC PENICILLIUM SPECIES ISOLATED FROM SALVADORA SPECIES Authors: KOREJO, FARZANA; ALI, SYED ABID; SHAFIQUE, HAFIZA ASMA; SULTANA, VIQAR; ARA, JEHAN; HAQUE, SYED EHTESHAMUL Abstract: Salvadora persica and S. S.oleoides are facultative holophytic plants, well known as miswak, are traditionally used to ensure oral hygiene among Muslim people in Asian and African counties. Species of Salvadora have a number of proven pharmacological importance. Besides, terrestrial fungi endophytic fungi are also gaining importance for the isolation of bioactive compounds. In this study 74 samples (root, shoot and leaves) from S .persica and S. oleoides were examined for endophytic fungi, 22 samples showed presence of Penicillium spp., 48 were found positive for aspergilli, whereas 10 samples showed infection of Fusarium solani,4 were found infected with Macrophomina phaseolina and one with Rhizoctonia solani. Most of the Penicillium isolated were identified as P. restrictum, P. citrinum and P. canescens. In dual culture plate assay out of four Penicillium isolates tested, P.citrinum and one isolate of P.restrictum caused growth inhibition of all four test root rotting fungi, Fusarium solani, F. oxysporum, Macrophomina phaseolina and Rhizoctonia solani. Culture filtrates of Penicillium spp., were also evaluated against four common laboratory bacteria namely Bacillus subtilis, Staphylococcus aureus, Salmonella typhimurium and Escherichia coli and above mentioned root rotting fungi. Culture filtrates of endophytic Penicillium spp., also showed significant antibacterial and antifungal activity. Secondary metabolites of endophytic Penicillium spp., offer an exciting area of research for the discovery of novel antimicrobial compounds. 2014-02-20T00:00:00Z http://localhost:80/xmlui/handle/123456789/14818 Title: COMPARATIVE EFFICACY OF DIFFERENT FUNGICIDES AGAINST FUSARIUM WILT OF CHICKPEA (CICER ARIETINUM L.) Authors: MAITLO, S.A.; SYED, R.N.; RUSTAMANI, M.A; KHUHRO, R.D.; LODHI, A.M Abstract: Fusarium wilt caused by Fusarium oxysporum f. sp. ciceris (Foc) is the most serious and widespread disease of chickpea, causing a 100% loss under favorable conditions. Fourteen fungicides were evaluated against wilt pathogen In vitro with five different concentrations ranging from 1-10000 ppm. Among these only Carbendazim and Thiophanate-methyl was found as the most effective at all used concentrations. Other fungicides like Aliette, Nativo, Hombre-excel and Dividend star were found to be moderately effective. Whereas, remaining fungicides were ineffective against the targeted pathogen. Generally, a positive co-relation was observed between increasing concentrations of the tested fungicides and inhibition of Foc. Based on In vitro results Carbendazim, Thiophanate-methyl, Aliette, Dividend-star, Hombre-excel, Score and Nativo were selected for pot and field experiments. The higher concentrations of the few fungicides completely inhibited the pathogen as well as found to be phytotoxic and suppressed the plant growth while lower concentrations promoted the growth of chickpea plants. On over all bases, the Carbendazim and Thiophanate-methyl, followed by Aliette and Nativo were more effective in reducing the impact of pathogen as well as enhancing the plant growth in greenhouse experiment. Under field conditions, all fungicides except Score remarkably decreased the disease development and subsequently increased the plant growth as well as grain yield as compared to untreated plants. 2015-01-19T00:00:00Z http://localhost:80/xmlui/handle/123456789/14816 Title: MORPHO-MOLECULAR IDENTIFICATION OF A NOVEL ASPERGILLUS SPP. AND ITS CULTURAL OPTIMIZATION FOR LIPASES PRODUCTION Authors: IFTIKHAR, TEHREEMA; NIAZ, MUBASHIR; HAIDER, MUHAMMAD ZULQURNAIN; SIDRA, SIDRA; DURR-E-NAYAB, DURR-E-NAYAB; ABDULLAH, ROHEENA; ZIA, MUHAMMAD ANJUM Abstract: Different lipid rich products were used to obtain oil degrading fungal isolates. The isolates were codified for referral to our culture bank and compared for their lipolytic potential. Amongst the isolates, MBL-1412 isolated from the cooked “sliced cicer arietinum” (Channa Daal) was found to be a potent hyper-producer and was optimized for lipase production under solid state fermentation. Initial systematic treatment based upon micrometric data and consultation with the standard monographs and fungus ended up with its identification as Aspergillus sp. The identification confirmed that the fungus belongs to genus Aspergillus, by DNA barcoding marker like 18S rRNA gene sequence.Later, the sequence was registered with accession no. KM924434 in the public nucleotide library (genbank) of NCBI. Fungal culture was maintained on 2% potato dextrose agar (PDA) during the study. Diverse substrates of agricultural byproducts under varied incubation temperature, time interval, inoculum level and different pH of diluent were used as parameters of optimization for hyperproduction of lipases. Different carbon and nitrogen sources as additives of culture medium were applied for enhancement of lipase production. Almond meal (10g) with inoculum level @1.5 mL after 48 h of time course at 50ºC and 6 pH were selected to be the best eco-cultural conditions for optimal lipases production by Aspergillus sp. MBL-1412. Supplementary additives of nitrogen and carbon sources to the basal substrate improved lipases production appreciably. Ammonium chloride (1%) as inorganic nitrogen source, nutrient broth (0.8%) as organic nitrogen source and starch (0.8%) as carbon source were found as best media additives for enhanced extracellular lipases yield. 2015-01-18T00:00:00Z