http://localhost:80/xmlui/handle/123456789/14690 2026-04-23T03:15:34Z http://localhost:80/xmlui/handle/123456789/14984 Title: Isolation and identification of endophytic fungi from Dendrobium huoshanense with their antibacterial and anti-inflammatory activities Authors: Yue, Jing-Yi; Yu, Yang Abstract: To examine the symbiotic relationship between Dendrobium huoshanense and its endophytic fungi, a total of 168 endophytic fungi were isolated and classified into 18 genera and 36 species via using pure culture and techniques of molecular biology. Among them, Stagonosporopsis oculihominis, Alternaria eichhorniae, Phyllosticta aristolochiicola, Aspergillus flavus and Fusarium lactis were the dominant genera. Further, the secondary metabolites produced by the dominant genera were screened for antibacterial and anti-inflammatory activities. The secondary metabolites of Stagonosporopsis oculihominis showed good inhibitory activity against Staphylococcus aureus and the release of nitric oxide (NO). Three benzene ring derivatives were also identified using chromatographic and spectroscopic techniques, including (Z)-1- hydroxy-4-(2-nitroethenyl)-benzene (1), p-hydroxybenzaldehyde (2) and p-hydroxyphenylacetic acid (3). These findings indicated that the endophytic fungi of Dendrobium huoshanense are diverse and their dominant genera exert similar anti-inflammatory and antibacterial effects as those of the host plant, which provided a scientific basis for the subsequent investigation of the medicinal value of Dendrobium huoshanense. 2022-07-20T00:00:00Z http://localhost:80/xmlui/handle/123456789/14983 Title: Optimization and fabrication of the nanosponge carriers of on dansetron using one-factor design Authors: Salawi, Ahmad; Alam, Mehwish; Zaman, Muhammad Abstract: The current studies were aimed to formulate ethyl cellulose (EC), beta-cyclodextrin βCD facilitated EC based Ondansetron nanosponges (NS) using Response Surface Methodology (RSM) by employing One Factor Design. The NS were fabricated by Emulsion Solvent Diffusion method, followed by characterizations including, drug-polymer compatibility, entrapment efficiency, percentage yield, zeta size, zeta potential and in-vitro release of drug and Scanning Electron Microscopy (SEM) and X-Ray Diffractometry (XRD). The outcomes of Fourier Transformed Infra-Red Spectroscopy (FTIR) have confirmed the compatibility of the drug and excipients. It was found that NS have good entrapment efficiency along with their satisfactory percentage yield. Particle size analysis has confirmed the synthesis of nanosized NS (87.8nm to 108.2nm), having spongy surface, that was described by SEM results. Furthermore, the drug release studies have described a good sustained release of ondansetron for the period of 8 hours. The kinetic modeling has predicted that drug would follow the non fickian type of diffusion mechanism. The application of statistical approach was found helpful in designing and evaluating the NS, avoiding the laborious work, needs to be conducted while using hit and trial method. 2022-07-20T00:00:00Z http://localhost:80/xmlui/handle/123456789/14982 Title: Screening and estimation of antibacterial activity of bacteriocins produced by Enterococcus faecium LR isolated from raw horse milk samples Authors: Kadi, Roqayah Hassan; Baghdadi, Afra Mohammed Abstract: Lactic acid bacteria are microorganisms that can be present in meat, milk and fermented products, as well as fermented drinks and vegetables, and they inhibit the growth of pathogenic and decaying microorganisms. Our aim was isolating, screening, characterization and estimation of bacteriocin/s of Lactic acid bacteria isolated from Horses milk in Jeddah city, Saudi Arabia. That have antagonistic activity against a wide range of pathogenic bacteria. On MRS agar medium 16 LAB isolates were examined using the agar well diffusion method against the pathogenic bacteria (Grampositive bacteria: S. aureus ATCCBAA977, ST. Pneumonia ATCC49619) and (Gram-negative bacteria: E. coli ATCC35218, P. aeruginosa ATCC27853). The results showed that 12 isolates out of 16 isolates (75%) worked to inhibit all types of pathogenic bacteria used. The best isolate was identified using molecular methods; the results showed that the isolate (H6) matched 100% with (Enterococcus faecium LR135401.1). This isolate produced the highest bacteriocin at 35°C/24 h/pH (6.5). 2022-07-20T00:00:00Z http://localhost:80/xmlui/handle/123456789/14981 Title: Synthesis, biological screening and docking studies of some indole derivatives as potential antioxidant Authors: Ansari, Sumaira; Mushtaq, Nousheen; Ahmed, Ahsaan; Asghar, Saira; Munawar, Rabya; Saify, Zafar Saied Abstract: The present study envisioned some antioxidant candidates having 1,2,3,4-tetrahydro-9H-pyrido[3,4-b]indole (TRY), 3-(2-bromoethyl)indole (BEI) and 7-azindole (AI) nucleus. Derivatives of these indole molecules were synthesized and their scavenging activity for reactive oxygen species (ROS) investigated by 2,2-diphenyl-1picrylhydrazyl (DPPH) radical scavenging assay. T3, T42 exhibited significant radical scavenging potential which is comparable to ascorbic acid (standard), while T36 appeared as most potent antioxidant by displaying better scavenging activity than standard molecule. Molecular docking study revealed good binding score and interactions of T36 with target human antioxidant enzyme (PDB code: 3MNG) validating the results of biological activity. 2022-07-20T00:00:00Z