http://localhost:80/xmlui/handle/123456789/17140 2026-04-19T06:10:19Z http://localhost:80/xmlui/handle/123456789/18072 Title: COCONUT' ABSCESS IN A BUFFALO Authors: Muhammad Athar; G. Muhammad; Abdul Sbakoor Abstract: Case History and Clinical Examination A 7-years old Nili-Ravi buffalo in her 3'd lactation was brought to the outdoor clinics of Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, for the treatment of a massive swelling at the base of left ear. Clinical examination revealed a coconut-shaped hard hanging mass attached to the posterior aspect of the left aural base (Plate 1). Anamnesis indicated that the mass had been steadily growing for the last four years. The mass was painless, pendulous; had a definite neck (5 x 10 cm) and body (20 cm circumference) (Plate 2). The upper third of the body of the mass had a doughy consistency whereas the rest of the body was covered with long superficial dry fissures traversing along its long axis. The vital parameters (temperature, pulse and respiration) of health were within normal ranges for buffalo (Cockrill, 1974). Feed intake and milk yield had remained unaffected eversince the mass was noted. Exploratory puncture with a sterile 16 gauge (2.5 cm long) revealed thick tapioca-like granular pus in the centre of the mass. As such, a diagnosis of chronic abscess was arrived at. Culturing of the pus on the blood agar containing 5% sheep erythrocyte revealed multiple 13-haemolytic colonies of Staphylococcus uureus in pure culture. Surgical Treatment and Response The animal was sedated by intramuscular injection of 5 mI Sedastress (Acepromazinc 10 mg/m1). The cord-like base of the mass was prepared aseptically for surgical management. The base of the neck was anaesthetized by infiltrating xylocaine hydrochloride 2% around it. The incision was made at its base to expose the cord-like root containing blood supply which was ligated with chromic catgut No. I and was excised followed by closure of one inch long incision with braided surgical silk No. 2. The mass weighed 3 Kg. Sawing the mass revealed thick fibrous capsule encircling a small amount of thick granular non-ordourous light brown pus of the texture of cream of wheat. Injection Combiotic 5 g (Strepto-pencillin) IM daily for 4 days was given as post-operative measure. Wound was dressed aseptically with Tr. Iodine until its complete healing followed by removal of stitches on day 10 post-operatively. The recovery was uneventful. 1999-07-13T00:00:00Z http://localhost:80/xmlui/handle/123456789/18071 Title: USE OF DOT-ELISA FOR THE DIAGNOSIS OF INFECTIOUS BURSAL DISEASE IN CHICKENS Authors: Jawaria Sultana; Manzoor Hussain; Malik, Salman A.; Khalid Naeem Abstract: The present study was undertaken to develop dot-blot ELISA (DOT-ELISA), a field oriented diagnostic test to detect infectious bursal disease virus (IBDV). The DOT-ELISA, using nitrocellulose membrane, was standardized and field specimens collected from sick birds, apparently infected with IBDV, were analyzed. Efficacy of DOT-ELISA was compared with agar gel precipitation test (AGPT). The DOT-ELISA was found to be more sensitive diagnostic tool than AGPT, indicating the suitability of DOT-ELISA as rapid field test for the detection of IBDV during early stage of infection, 1999-07-12T00:00:00Z http://localhost:80/xmlui/handle/123456789/18069 Title: INDIRECT HEMAGGLUTINATION TEST FOR THE DETECTION OF ANTIBODIES AGAINST BOOPHILUS MICROPLUS Authors: C.S. Hayat; Masood Akhtar; Iftikhar Hussain; Rehan Ahmad Abstract: Indirect hemagglutination (IHA) test for the detection of antibodies against Boophilus (B.) microplus was developed. Midgut, B. microplus, antigen was used to sensitize the human group "0" erythrocytes without coupling agent. The optimum temperature for the sensitization of erythrocytes was found to be 37°C. The sensitizing dose of the antigen was calculated as 2.0 mL per 0.01 mL of the packed erythrocytes, diluted in 2 mL phosphate buffered saline, at 37°C for 60 minutes. The IHA results were best seen after 40 minutes of adding the sensitized erythrocytes in the serially diluted samples. IHA was found to be simple, rapid and inexpensive test for the detection of anti-tick antibodies. 1999-07-11T00:00:00Z http://localhost:80/xmlui/handle/123456789/18068 Title: SOME EPIDEMIOLOGICAL ASPECTS OF MASTITIS IN COWS AND BIOCHARACTERIZATION OF ISOLATED STAPHYLOCOCCI Authors: Javed lqbal; M. Siddique Abstract: Among one thousand cows, 220 animals were positive for mastitis thus the point prevalence being 22 per cent. Of the total milk samples, 486 (12.21%) were positive by White side test and 10.93 per cent by pH indicator paper technique. Incidence was higher in hind quarters as compared to the fore-quarters and slightly higher in right quarters than the left ones. Incidence of mastitis was the maximum(23.19%) in crossbred cows and the minimum (7.69%) in nondescript indigenous breeds. Occurrence of mastitis increased with the increase in age and number of lactations.The disease was more prevalent in cows during the first month of lactation (24.90%). Prevalence of mastitis was higher in farm than in field conditions. The occurrence of mastitis was lower in cows where washing of udders was carried out prior to milking. Suckling of calf showed a nonsignificant effect. A total of 190 milk samples were positive for bacterial isolations. Various isolated pathogenic microorganisms were Staphylococcus aureus (32.09%), coagulase negative staphylococci (12. 06 % ), E. coil (16.o8), Streptococcus agalactiae (3. 01 % ), Streptococcus uberis (7%), Pseudomonas aeruginosa (7.5%), Corynebacteriwn pyogettes (3.51%), Bacillus (3.01%) and Klebsiella (2.01 %). Total fungal isolates were 1 1 , among which 9 were Candida and 2 were Aspergillus. The staphylococci were highly sensitive to oxytetracycline , sulphamethaxazole/ trimethopritu and chloramphenicol, while streptococci were highly sensitive to oxytetracycline, erythromycin and chloramphenicol. E.coli was sensitive to oxytetracycline , gentamicin, chloramphenicol and streptomycin. All DNAse positive Staphylococcus aureus strains were coagulase positive. Penicillin resistance was showed by 49.24 per cent Staph. aureus cultures and 50 per cent coagulase negative staphylococcus strains. Out of 32 penicillin resistant strains of Staphylococcus aureus, 26 (81.25%) were resistant due to penicillinase production. 1999-07-10T00:00:00Z