DSpace Collection:

IN SILICO BASED DEVELOPMENT OF dLUTE LENGTH POLYMORPHISM MARKER FOR COMMON FLAX GERMPLASM EVALUATION

Tue, 24 Nov 2015 00:00:00 GMT

Title: IN SILICO BASED DEVELOPMENT OF dLUTE LENGTH POLYMORPHISM MARKER FOR COMMON FLAX GERMPLASM EVALUATION Authors: ŽIAROVSKÁ, JANA; BEŽO, MILAN; LANCÍKOVÁ, VERONIKA; RAŽNÁ, KATARÍNA Abstract: The first study of the pattern of dLUTE insertions as detected by PCR length polymorphism technique was performed. In silico approach was used for developing the dLUTE based marker system. Alignment analysis were performed to find a region that is dLUTE specific and primers designed withing this region not corresponding to any of sequences of L-genes. Realized length polymorphism analysis of common flax accessions confirmed the results of In silico analysis of polymorphic potential of designed marker system. The PCR analyse based of flax dLUTE transposon resulted in ten different alleles amplified in PCR withing the length range from 250 up to the 980 bp. In total, twenty-four common flax accessions were evaluated using the developed approach and the grouping of them according the oil/fiber type was obtained in constructed dendrogram.

MECHANISMS OF COMBINED EFFECTS OF SALT AND ALKALINE STRESSES ON SEED GERMINATION AND SEEDLINGS OF MELILOTUS OFFICINALIS (FABACEAE) IN NORTHEAST OF CHINA

Mon, 23 Nov 2015 00:00:00 GMT

Title: MECHANISMS OF COMBINED EFFECTS OF SALT AND ALKALINE STRESSES ON SEED GERMINATION AND SEEDLINGS OF MELILOTUS OFFICINALIS (FABACEAE) IN NORTHEAST OF CHINA Authors: SOAN VU, THI; ZHANG, DAWEI; XIAO, WEIHUA; CHI, CHUNYU; XING, YI; FU, DONGDONG; YUAN, ZENING Abstract: In line with the salt-alkalinized soils found in the northeast of China, the conditions were simulated to investigate the mechanisms associated with this combination of stresses on Melilotus officinalis. The effects of salinity (NaCl: 0-300mM) in combination with alkali (pH: 7.1-9.8) on the seed germination and seedlings of M. officinalis were investigated. The results showed that germination was not inhibited completely by the salt-alkali conditions tested. The recovery germinations were significant higher than the control or had no significant differences with the control under the conditions of NaCl<200mM and pH=9.0, suggesting that non-germinated seeds may have a strategy to get through and resist the stress during germination stage. For the seedling growth, M. officinalis was capable of surviving at high pH (pH≤9.8) and the salinity (NaCl≤200mM) (seedling survival rate: 84.77±8.62%). The characteristic feature for combined salt-alkali stresses is the reciprocal enhancement between salt and alkali stresses. The combined action of salinity and pH should be considered when evaluating the effects of salt-alkali stresses. Correlation and regression analyses showed that salinity was the dominant stress factor, while pH was a secondary factor. From the physiological and ecological parameters, we suggested that M. officinalis is a salt-alkali tolerant species which can be used in vegetative restoration of saline soils in the northeast of China.

AN IMPROVED, SIMPLE, INEXPENSIVE AND HIGHLY FLEXIBLE HYDROPONIC SETUP FOR ROOT MITOCHONDRIA ISOLATION FROM ARABIDOPSIS AND NICOTIANA PANTS

Sun, 22 Nov 2015 00:00:00 GMT

Title: AN IMPROVED, SIMPLE, INEXPENSIVE AND HIGHLY FLEXIBLE HYDROPONIC SETUP FOR ROOT MITOCHONDRIA ISOLATION FROM ARABIDOPSIS AND NICOTIANA PANTS Authors: WAQAR HAMEED, MUHAMMAD; UD-DIN, AZIZ Abstract: Hydroponic setups are frequently developed and improved as they are convenient platforms for studying whole plant physiology. Mostly, the available systems produce small amounts of plant material and are therefore, unsuitable for studies requiring large quantities of plant material like isolation of mitochondria. To address this issue, we have modified a hydroponic setup that can sustain hundreds of Arabidopsis and tobacco plants until adult plants are established. The setup is very flexible and easy to construct. It is based on the use of recyclable and sterilizable plastic-net-pots and media containers, which are easily available from the local suppliers. The modified seed-pots and styrofoam sheets facilitate the transfer and harvesting of seedlings. We have used the Percoll based two-step density gradient centrifugation method for the isolation of root mitochondria from the hydroponically grown plants.

ANTIBACTERIAL ACTIVITY OF FUMARIA INDICA (HAUSSKN.) PUGSLEY AGAINST SELECTED BACTERIAL STRAINS

Sat, 21 Nov 2015 00:00:00 GMT

Title: ANTIBACTERIAL ACTIVITY OF FUMARIA INDICA (HAUSSKN.) PUGSLEY AGAINST SELECTED BACTERIAL STRAINS Authors: TOOR, YASMIN; NAWAZ, KHALID; HUSSAIN, KHALID Abstract: Antibacterial properties of methanolic extracts of F. indica prepared in different doses against seven Gram-positive and Gram-negative bacterial strains i.e. Streptococcus pyogenes, Staphylococcus aureus (1), Staphylococcus aureus (2), Shigella sonnei, Escherichia coli (1), Escherichia coli (2) and Neisseria gonorrhoeae using agar well diffusion method (inhibition zone measurements) compared to gentamicin as standard antibiotic. Results showed significant activities against the test organisms with overall satisfactory statistics. Streptococcus pyogenes, Staphylococcus aureus strains as well as Neisseria gonorrhoeae showed more inhibition to methanolic extracts of F. indica. Minimum inhibitory as well as minimum bactericidal concentrations against all strains except Shigella sonnei were also recorded. Studies showed promising horizons for the use of F. indica as an active antibacterial component in modern drug formulations.