http://localhost:80/xmlui/handle/123456789/13182 Thu, 23 Apr 2026 17:34:24 GMT 2026-04-23T17:34:24Z http://localhost:80/xmlui/handle/123456789/13293 Title: Spectroscopic interactions of non-insulin-dependent diabetes mellitus with levocetirizine Authors: Mehboob, Shafaque; Mehboob, Moona; Mehjabeen; Tariq Rafi, SM; Khan, Tariq; Anser, Humaira; Perveen, Shaheen; Jahan, Noor; Wazir, Asma; Owais, Farah Abstract: Non insulin dependent diabetes mellitus (NIDDM) drugs such as glibenclamide and metformin is employed to heterogeneous disorder characterized by alteration in production of glucose due to impairment of both insulin secretion and insulin action. These patients might suffer with allergic rhinitis and in this case, there is a possibility to maintain patient on levocetirizine, an anti-allergic drug commonly used in rhinitis. The object of the present study is to detect possible interaction between glibenclamide or metformin with levocetirizine Current study was performed using UV spectroscopic technique sing simultaneous equation in pH simulated to gastric juice (pH 1), pH 4, pH 7.4 and in pH 9. All drugs followed Beer Lambert’s Law. Results showed that glibenclamide and metformin can increase or decrease availability of levocetirizine and in the same way levocetirizine can alter availabilities of glibenclamide and metformin in different pH. Hence, drug interaction between glibenclamide or metformin with levocetirizne occurred. This may be due to his may be due to the charge transfer or binding capabilities of these drugs which resulted in significantly changed availability of NIDDIM as well as levocetirizine. Therefore, co-administration of these drugs should be avoided and furtherinvestigations at clinical and pre-clinical levels should be done. Tue, 20 Jul 2021 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/13293 2021-07-20T00:00:00Z http://localhost:80/xmlui/handle/123456789/13292 Title: Development and validation of single analytical HPLC method for determination of flavoxate HCl in bulk, tablets and biological fluids Authors: Rashid, Ahmad Junaid; Bashir, Sajid; Bukhari, Nadeem Irfan; Abbas, Nasir; Raza, Atif; Munir, Ans; Ijaz, Qazi Aamir; Akbar, Shehla; Arshad, Numera; Ishtiaq, Saiqa Abstract: A simple, sensitive and precise high performance liquid chromatographic (HPLC) method was developed and validated for determination of flavoxate HCI in raw material, tablets and biological fluids. The method followed by using the Zorbax XDB-C18 column containing Di-isobutyl n-octadeceylsilane (4.6mm×150mm, 5μm). The mobile phase consisted of acetonitrile: methanol: 0.15M sodium perchlorate (17:35:48 v/v) having pH 3. UV detection was carried out at 229nm at 40°C. Results indicated that the method has successfully established and validated in accordance with ICH guidelines acceptance criteria for linearity (0.03-7.5µg), accuracy (101.18-101.28%), robustness of column age and column lot (peak area %CV≤0.04, purity %CV≤ 0.006) and robustness of HPLC condition (%CV≤ 0.02), precision (intra and inter day precision assay, %CV values for peak area and percent purity of flavoxate HCl≤2%) and system suitability parameters. The average noise, theoretical LOD and LOQ were found to be 0.01 mAU, 0.03 mAU and 0.6ng, respectively. The Coefficient of determination (r2 ) ranging from 0.03µg to 7.5µg, 0.99 which was within acceptable criteria of r2 & gt 0.99. The spiked recoveries of samples were 101.28, 101.18 and 101.18% respectively. All data revealed that this method can be used for in-vitro & in-vivo determination of flavoxate HCI in various pharmaceutical preparations. Tue, 20 Jul 2021 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/13292 2021-07-20T00:00:00Z http://localhost:80/xmlui/handle/123456789/13291 Title: Development of grape seed extract based formulations by using noninvasive biophysical technique and its impact on skin aging Authors: Rafique, Misbah; Shah, Syed Nisar Hussain; Hussain, Irshad; Javed, Imran; Nisar, Naveed; Riaz, Romana Abstract: Given the substantial benefits of grape seed extract (GSE) in reducing oxidative stress, the study aimed development, characterization and comparative analysis of GSE-based formulations. The development entailed extraction of GSE from Vitisvinifera L. HPLC confirmed catechin, epicatechin, gallic acid, epicatechingallate and procyanidin dimers. Storage of Formulations observed, Stability & rheological parameters determined. Olive oil used as a permeability enhancer. Presence of the highest oleic acid content (65-86%) in Olive oil, skin permeability within the stratum corneum was enhanced hence better transdermal skin absorption. Using two-way ANOVA, and T-test, efficacy of formulations and impact on slowing down skin aging by countering exogenous factors of oxidative stress determined. Non-invasive biophysical technique showed emulgel substantially reduced roughness, scaliness, winkles, and sebum content by 55%, 26 %, 23.9% and 30.3% respectively enhancing elasticity and hydration by 50% and 32.2% respectively. Emulsion reduced roughness, scaliness, winkles and sebum content 14%, 13%, 21% and 26.13% respectively enhancing elasticity and hydration 45.3% and 29.85% respectively. The formulations significantly offset exogenous factors of aging and impact on free radicals and oxidative stress and may be safe to incorporate bio-active botanical antioxidants for evaluation of derma cosmetic benefits in management of dehydrated and aged facial skin. Tue, 20 Jul 2021 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/13291 2021-07-20T00:00:00Z http://localhost:80/xmlui/handle/123456789/13290 Title: Neuroprotective role of a monoterpene (thymol) on diazepam induced withdrawal symptoms in rats Authors: Saleem, Sadia; Naqvi, Fizza; Batool, Asma; Naqvi, Sajjad Haider; Naqvi, Faizan; Batool, Zehra; Tabassum, Saiqa; Haider, Saida Abstract: : Benzodiazepine administration is known to be related to tolerance and a withdrawal syndrome on sudden cessation. Thymol possesses multiple biological properties especially in the pathogenesis of different brain disorders. However, to the best of our knowledge there is no study that relates the use of thymol to benzodiazepine induced withdrawal symptoms. Therefore the aim of the current study was to investigate the usefulness of thymol in the treatment of benzodiazepine withdrawal syndrome in rats. Animals were divided into four groups, thymol (40 mg/kg/ml), diazepam (4 mg/kg), thymol + diazepam and vehicle control group. The treatment was given for 14 days, and then suddenly ceased. After 24 h animals were tested in different behavioral paradigms such as physical signs for withdrawal, marble burying test, inverted screen test, elevated plus maze, passive avoidance test and open field activity. The results of the present study revealed that co-administration of thymol significantly reduced the withdrawal symptoms induced by diazepam. Our results further suggest that administration of thymol not only ameliorates rebound anxiety associated with diazepam withdrawal but also improves motor and memory impairment in rats. Tue, 20 Jul 2021 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/13290 2021-07-20T00:00:00Z