http://localhost:80/xmlui/handle/123456789/14036 Thu, 23 Apr 2026 09:41:02 GMT 2026-04-23T09:41:02Z http://localhost:80/xmlui/handle/123456789/15665 Title: THE ASSESSMENT OF GENETIC DIVERSITY BETWEEN AND WITHIN BRASSICA SPECIES AND THEIR WILD RELATIVE (ERUCA SATIVA) USING SSR MARKERS Authors: MEHWISH KANWAL; FARHATULLAH; M. ASHIQ RABBANI; SIDRA IQBAL; LAILA FAYYAZ; IFFAT NAWAZ Abstract: Microsatellites markers were tested for their ability to distinguish genomic distribution of the Brassica species of the U Triangle and E. sativa. The objectives of the present study were to investigate the genetic diversity of six Brassica species from U-Triangle (representing three genomes, A, B, C) and one from genus Eruca and to identify promising sources of genetic variation for breeding purposes. A total of 54 SSR markers were analyzed in order to detect variation between and within the selected genomes. Three primer pairs depicted the greatest genetic diversity showing 97% polymorphism between Brassica and Eruca genomes (2.55 alleles per locus). Polymorphic Information Content (PIC) values ranged from 0.40 (SSR primer Na14-DO7) to 0.79 (NA10-G09). For comparison within Brassica genomes and Eruca, all the genomes were grouped in three modules i.e., ABE, ACE and BCE (Fig. 1). The tetraploid originating from their parental diploids alongwith Eruca was considered in the same module. For the estimation of relatedness within and among genomes, dice coefficients were computed as a measure of genetic similarity matrix. On the basis of genetic distances, dendrogram was constructed through cluster analysis. Two major clusters at coefficient of similarity level (0.47) were observed. One cluster comprised of all Brassica genomes and their accessions, while another consisting of all accessions of Eruca genome. The cluster containing Brassica genomes was further subdivided into four sub-groups that contained diploid and tetraploid species in a way that tetraploid species were grouped in between their diploid parental species with varying genetic distances. Present findings confirmed the validity of SSR markers in genomic studies. Wed, 17 Sep 2014 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/15665 2014-09-17T00:00:00Z http://localhost:80/xmlui/handle/123456789/15664 Title: PLANT REGENERATION FROM IN VITRO-SELECTED SALT TOLERANT CALLUS CULTURES OF SOLANUM TUBEROSUM L. Authors: ZAHOOR AHMAD SAJID; FAHEEM AFTAB Abstract: This research work reports In vitro direct selection of salt-tolerant callus cultures and subsequent plant regeneration in two potato cultivars (Cardinal and Desiree). Results have shown more than 50% reduction in relative fresh callus mass in the two potato cultivars exposed to 120 mM NaCl. Callus morphology correspondingly changed from off-white to blackishbrown at 120 mM to acutely-necrotic at 140 mM NaCl. Regeneration potential of recurrently-selected callus cultures (100 mM NaCl-treated) on salt-free regeneration medium (MS + 2.64 µM NAA and 1.00 µM TDZ) was not much different as compared to the control (non-selected ones). Regenerated plants from salt-tolerant callus cultures of both the cultivars after selection were transferred to soil and organic matter (50:50, v/v) for acclimatization in the greenhouse. It was observed that the recurrently-selected plants had higher fresh/dry weight and number of tubers compared with the control ones in both the cultivars. Likewise the protein, peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) activities have shown an increasing trend in salt-treated plants of both the cultivars. The results from this study highlighted a strong possibility for the selection of salt-tolerant callus lines of potato followed by an efficient plant regeneration and further acclimatization. Tue, 16 Sep 2014 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/15664 2014-09-16T00:00:00Z http://localhost:80/xmlui/handle/123456789/15663 Title: TAXONOMIC STUDIES ON THE GENUS COSMARIUM CORDA (DESMIDOPHYCEAE) FROM NORTH-EASTERN AREAS OF PAKISTAN Authors: A. ZARINA; FARIHA NAZ; MUSTAFA SHAMEEL Abstract: Forty-one species of the algal genus Cosmarium Corda ex Ralfs (phylum Volvophycota Shameel) were collected from various freshwater habitats in Azad Kashmir as well as provinces of the Punjab and Khyber Pakhtoonkhwa of Pakistan during January 2003 and December 2006. Out of these 11 species were taxonomically investigated and described here. Among them C. pulchrum Turner, C. repandum Nordstedt, C. rugosum Turner, C. tetraophthalmum Brébisson in Ralfs, C. tumidum P. Lundell are being reported for the first time from Pakistan. Mon, 15 Sep 2014 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/15663 2014-09-15T00:00:00Z http://localhost:80/xmlui/handle/123456789/15662 Title: MORPHO-ANATOMY OF STYPOPODIUM ZONALE (PHAEOPHYCOTA) FROM THE COAST OF KARACHI, PAKISTAN Authors: ALIA ABBAS; MUSTAFA SHAMEEL Abstract: A brown alga Stypopodium zonale (Lamouroux) Papenfuss (Dictyotales) was collected from Manora and Buleji, the coastal areas near Karachi (Pakistan) during March 2006-April 2009 and investigated for its morphology, anatomy and reproductive structures. This is the first detailed study on the Pakistani specimens of this species from these points of view, where presence or absence of intercellular spaces, cell-wall thickness of different cells and structure of surface cells were examined. In this connection the apical, middle and basal parts of the thallus were investigated anatomically. Sun, 14 Sep 2014 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/15662 2014-09-14T00:00:00Z