http://localhost:80/xmlui/handle/123456789/14470 Thu, 23 Apr 2026 12:43:50 GMT 2026-04-23T12:43:50Z http://localhost:80/xmlui/handle/123456789/15860 Title: CHROMOSOME LOCATION AND SSR MARKERS OF A STRIPE RUST RESISTANCE GENE FROM WHEAT (TRITICUM AESTIVUM) LINE N9738 Authors: YANG, BAOJU; JI, WANQUAN; WANG, CHANGYOU; XUE, FEI; M. ALI; WANG, YAJUAN; ZHANG, HONG; LIU, XINLUN Abstract: The objective of present study here was to determine the inheritance and chromosomal location of the resistance gene in N9738. The phenotypic data of F2 plants, from the crosses N9738/Huixianhong and N9738/Abbondanza, respectively, implied that the stripe rust resistance in N9738 was controlled by a single dominant gene, temporarily designated YrN9738. Monosomic (Nullisomic) analysis revealed YrN9738 is on wheat chromosome 1B. Seventy-eight SSR markers on chromosome 1B were screened on the parents and bulks. Four SSR markers (Xgpw7422, Xgpw5195, Xgpw7812, and Xcfd65) were used to construct a linkage map for YrN9738 and Xcfd65 was closely linked to YrN9738 with genetic distance of 3.5 cM. These linked markers were located on chromosome 1BL with Chinese Spring nullisomic-tetrasomics and ditelosomics of homoeologous group 1. Their presence in the deletion stocks of chromosome 1B located Xgpw7422 and Xgpw7812 in bin 1BL06-0.32-0.47 and Xgpw5195 in bin 1BL06-0.32. YrN9738 should be on wheat chromosome 1B near to the centromere and useful in wheat breeding programs. Sun, 02 Jun 2013 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/15860 2013-06-02T00:00:00Z http://localhost:80/xmlui/handle/123456789/15859 Title: STUDY ON CHROMOSOMAL STRUCTURAL HETEROZYGOSITY IN PAEONIA EMODI, AN ENDANGERED SPECIES Authors: WANG, SHI-QUAN Abstract: Chromosomal meiotic behavior in pollen mother cells (PMCs) of Paeonia emodi, an endangered species, was investigated in natural population represented by nine individuals. The results showed that: (1) Mean chromosome configuration was 2n = 10 = 0.20 I + 4.90 II at metaphase. Most of the chromosomes were ring bivalents, but some were rod bivalents or univalents. The existence of rod bivalent and univalent indicated the differentiation between the homologous chromosomes, most probably due to chromosomal structural heterozygosity. (2) All individuals studied were chromosomal structural heterozygotes, i.e., inversion, as indicated by bridge and/or fragment. (3) There were some variations among individuals in size of fragments, which indicated that different inversions existed in this species, i.e., paracentric inversion polymorphisms. Chromosomal structural heterozygosity is a common phenomenon in Paeonia. Further research is necessary to probe into the reasons that structural heterozygosity exists widely, and whether there is relationship between heterozygosity and ecological adaptation in this species. Sat, 01 Jun 2013 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/15859 2013-06-01T00:00:00Z http://localhost:80/xmlui/handle/123456789/15799 Title: COMPARATIVE EFFECTS OF NACL AND SEA SALT ON SEED GERMINATION OF TWO HALOPHYTIC GRASSES UNDER VARIOUS LIGHT AND TEMPERATURE REGIMES Authors: FAIZA SHAIKH; BILQUEES GUL; RAZIUDDIN ANSAR; ABDUL REHMAN A. ALATAR; AHMED. K. HEGAZY; M. AJMAL KHAN Abstract: Effect of several concentrations of NaCl or sea salt was studied on seeds germination of two halophytic grasses- an annual (Eragrostis ciliaris) and a perennial (Dichanthium annulatum) in controlled growth chambers maintained at 10:20, 15:25, 20:30 and 25:35o C (12 h dark: light) and 24 h dark photoperiods. Sea salt and NaCl both inhibited seed germination but NaCl prevented more seed from germination. Optimal germination was obtained at temperature regime of 25:35o C for both grasses. Seeds of D. annulatum had similar germination both in light and dark at cooler temperatures in distilled water while addition of salinity in dark inhibited more seed germination compared to light treated seeds. No seeds of E. ciliaris germinated in dark and this positive photoblastic response was associated with temperature irrespective of salinity treatment. All seeds recovered from salts stress when returned to distilled water. Most of the un-germinated seeds were dormant and viable at the end of experiment indicating that they could form a viable seed bank to ensure the continuity of the population in saline habitat. Sun, 06 Jan 2013 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/15799 2013-01-06T00:00:00Z http://localhost:80/xmlui/handle/123456789/15798 Title: MOLECULAR CHARACTERIZATION OF SOIL BACTERIA FOR IMPROVING CROP YIELD IN PAKISTAN Authors: RIFAT HAYAT; RABIA KHALID; MUHAMMAD EHSAN; IFTIKHAR AHMED; YOKOTA, AKIRA; SAFDAR ALI Abstract: Nine Gram - positive bacterial strains designated as RH-1 to RH-9 were isolated from legumes rhizospheric soil and characterized for plant growth promoting (PGP) activities in legume and cereal crops as well as detailed morphological, phenotypic and biochemical studies. The strains were identified using 16S rRNA gene sequencing and chemotaxonomic traits. Based on comparative analysis of 16S rRNA gene sequencing, the strains showed highest similarity (97.9–99.8%) to the genus Bacillus. In vitro growth promoting assay indicated that the strain RH-5 proved potential PGPR by solubilizing highest amount of inorganic P (141 µg mL-1), positive for nifH gene (+) and produced indole acetic acid (IAA; 0.82 µg mL1 ) followed by strain RH-2. Phosphorus solubilization in broth culture was associated with significant drop in pH by the strain RH-5 from 7.0 to 4.8. These two strains were further evaluated for plant growth promotion in beans (mung bean; Vigna radiate, mash bean; Vigna mungo) and wheat (Triticum aestivium) under axenic condition. The strain RH-5 proved the best PGPR by increasing crops biomass and grains (wheat; 40-45%, beans; 50%) over un-inoculated control. The chemotaxonomic data (pre dominant menaquinone system: MK-7, DNA G + C content: 40-41 mol%, major cellular fatty acid: anteiso-C15:0) also supported the affiliation of both strains to the genus Bacillus. Phenotypic characterization showed that the strain RH-5 tolerated temperature: 50o C, pH: 10 and NaCl: 9%. Phylogenic and genotypic analysis showed the association of strains RH-5 and RH-2 with genus Bacillus having single phylitic lines to Bacilus gibsonii and Bacillus subtilis. Sat, 05 Jan 2013 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/15798 2013-01-05T00:00:00Z