http://localhost:80/xmlui/handle/123456789/16825 Thu, 23 Apr 2026 15:48:30 GMT 2026-04-23T15:48:30Z http://localhost:80/xmlui/handle/123456789/17540 Title: PRELIMINARY FLORISTIC LIST OF CHOTIARI WETLAND COMPLEX, NAWAB SHAH, SINDH, PAKISTAN Authors: Rahmatullah Qureshi; Ghulam Akbar Mon, 20 Apr 2009 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/17540 2009-04-20T00:00:00Z http://localhost:80/xmlui/handle/123456789/17538 Title: CHANGES IN MINERAL AND MINERALIZABLE N OF SOIL INCUBATED AT VARYING SALINITY, MOISTURE AND TEMPERATURE REGIMES Authors: Asma Lodhi; M.Arshad; F.Azam; M.H.Sajjad Abstract: A laboratory incubation experiment was conducted to study the effect of factors like moisture, salinity and temperature on the release of N in plant-available forms (NH4 and NO3+NO2-N) and potentially mineralizable N in soil over a period of 8 weeks following amendment with leguminous plant residues. In this experiment, soil samples salinized to ECe 7, 9, and 18 dS m-1 (original ECe was 5.0 dS m-1) were amended with 0.5% plant material of Sesbania aculeata and incubated at three moisture levels of 15, 30 and 45%, w/w and three temperature regimes of 20, 30 and 40oC for 8 weeks. Soil samples were sacrificed at 2, 4 and 8 weeks for the determination of NH4-N, NO3+NO2-N and mineralizable N. Ammonification of organic N as determined by the accumulation of NH4-N in soil was found to increase with time as salinity, moisture and temperature increased. However, the increase was more pronounced at higher moisture levels. While temperature had a positive effect on nitrification, increased salinity and moisture depressed the process. Net mineralization of N increased with time in all the treatments; the process being enhanced at higher incubation temperature with a maximum at 40oC. Salinity and high moisture had a depressing effect on the mineralization of N. The content of mineralizable N determined by NH4-N accumulation following 2 weeks of incubation under submerged conditions in soil remained higher under high moisture conditions, while high salinity and temperature had a variable and negative effect. Apparently, high moisture content conserved organic N due to reduced mineralization, while high temperature had an opposite effect. A complete loss of NO3-N was observed during incubation of soil samples for the determination of mineralizable N. This was attributable to denitrification as sufficient amount of easily oxidizable C was still present in the soil after 8 weeks of incubation under relatively aerobic conditions. Mon, 20 Apr 2009 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/17538 2009-04-20T00:00:00Z http://localhost:80/xmlui/handle/123456789/17537 Title: Pathogenesis of Pseudomonas syringae pv. sesami associated with sesame (Sesamum indicum L.) bacterial leaf spot. Authors: Firdous, Syed Sadiqa; Rehana Asghar; Irfan-ul-Haq, Muhammad; Abdul Waheed; Afzal, Syed Nadeem; Mirza, Muhammad Yasin Mon, 20 Apr 2009 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/17537 2009-04-20T00:00:00Z http://localhost:80/xmlui/handle/123456789/17535 Title: DEVELOPMENT OF MOLECULAR RESISTANCE IN POTATO AGAINST POTATO LEAF ROLL VIRUS AND POTATO VIRUS Y THROUGH AGROBACTERIUM-MEDIATED DOUBLE TRANSGENESIS Authors: M.Arif; P.E.Thomas; J.M.Crosslin; C.R.Brown Abstract: Potato leafroll virus (PLRV) and potato virus Y (PVY) are the two major viral problems for the potato production all over the world. Transgenic approaches involving the expression of viral genes are being developed to provide protection for plants against viral diseases. The purpose of this study was to develop double transgenic plants of potato using PLRV replicase and PVY coat protein genes tandemly placed in a single T-DNA transformant through Agrobacterium-mediated transformation. A total of 17 lines of putative transformants of potato cv. Desiree were generated from kanamycine resistant calli originated from co-inoculation of separate Agrobacterium cultures containing PVY CP and PLRV replicase genes. Shoots were excised and cultured onto shoot medium containing 250mg/L cefotaxime and 50mg/L kanamycin sulfate in test tubes. Polymerase chain reaction (PCR) analysis was conducted of 39 plants of 16 transformed lines using primers each of PVY CP and PLRV-replicase genes; 10 plants of 8 lines and 7 plants of 6 lines showed presence of of PVY CP and PLRV-replicase genes, respectively. However Mon, 20 Apr 2009 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/17535 2009-04-20T00:00:00Z