http://localhost:80/xmlui/handle/123456789/19283 Thu, 23 Apr 2026 00:18:56 GMT 2026-04-23T00:18:56Z http://localhost:80/xmlui/handle/123456789/19838 Title: Ethyl Chloroformate as Derivatizing Reagent for Gas Chromatographic Determination of α-Keto acids and Amino acids from Pharmaceutical Preparation Authors: KULSOOM UBEDULLAH ABBASI; MUHAMMAD YAR KHUHAWAR; MUHAMMAD IQBAL BHAMGER; KHALIDA PARVEEN MAHAR; ABDUL HAMEED CHANNAR Abstract: α-keto acids pyruvic acid (PYR), 2-ketobutyric acid (KBA), 3-methyl-2-oxobutyric acid (MKBA), 3- methyl-2-oxovalaric acid M3KVA), 4- methyl-2-oxovalaric acid (M4KVA), 2- ketohexanoic acid (KHA) and phenylpyruvic acid (PPYR) together with amino acids as derivatives of ethyl chloroformate (ECF) eluted and separated from GC column HP-5(30m x 0.32 mm id). The detection was by flame ionization ( (FID). Linear calibration curves were obtained within 18-127.8 µg/mL and detection limits were within 6-8.6 µg/mL of each α-keto acid. The separation was repeatable with inter and intraday variation with relative standard deviation (RSD) within 0.2-3.5%. A pharmaceutical preparation Ketosteril was analyzed and amounts of MKBA, M3KVA, M4KVA, and PPYR correlated with labeled values and their amount extracted from pharmaceutical preparation by standard addition were calculated to 93.92 -96.1 % with RSD 0.3-0.7%. The amino acids present in Ketosteril tablet separated completely and were determined simultaneously together with α-keto acids with recovery of 95.8 to 98.65% with RSD 0.2-0.9%. Sun, 30 Oct 2011 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/19838 2011-10-30T00:00:00Z http://localhost:80/xmlui/handle/123456789/19831 Title: Antiproliferative Activity and Chemical Constituents of Hypericum oblongifolium Authors: MUMTAZ ALI; MOHAMMAD ARFAN; KHAIR ZAMAN; HABIB AHMAD; NAZIA AKBAR; ITRAT ANIS; MUHAMMAD RAZA SHAH Abstract: The antiproliferative activity of hexane (F1), ethyl acetate (F2), butanol (F3) and water (F4) extracts of H. oblongifolium were tested in vitro for their anti- proliferative (anticancer) activity on the cell lines: HT-29 human colon adenocarcinoma, NCI-H460 human non-small cell lung carcinoma, MCF-7 human breast cancer, OVCAR-3 human ovarian adenocarcinoma and RXF-393 human renal cell carcinoma with etoposide as positive control. Among the various extracts the F1 showed relatively potent anti-proliferative activity (10.55 ± 4.19 µg/mL) on OVCAR-3 human ovarian adenocarcinoma cell growth almost equal to the activity of etoposide (9.42 ± 1.62 µg/mL). Seven compounds were also isolated for the first time from this source. These phytochemicals were identified as 18βH-urs-20 (30)-en-3β-ol-28-oic acid (1), tetracosyl 3-(3,4-dihydroxyphenyl) acrylate (2), Shikimic acid (3), 1-Octatriacontanol (4), Hexacosyl tetracosanoate (5), β-Sitosterol (6) and β-Sitosterol3-O-β-D-glucopyranoside (7) on the basis of spectroscopic studies such as 1H NMR ,13C NMR, 2D NMR and Mass spectroscopy as well as established with help of reported literature. Sat, 29 Oct 2011 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/19831 2011-10-29T00:00:00Z http://localhost:80/xmlui/handle/123456789/19830 Title: Antimicrobial and Antioxidant Activities of Phenolic Compound Extracted from New Verbascum Species Growing in Turkey Authors: FATMA ZERRIN SALTAN; MUNEVVER SÖKMEN; MEHTAP AKIN; HATICE TANER SARAÇOĞLU; RAMZAN SÜLEYMAN GÖKTÜRK; MANZOOR AHMAD; MUMTAZ ALİ; MUHAMMAD RAZA SHAH Abstract: The aim of this study was to evaluate the antimicrobial and antioxidant potential of the aerial parts of four new Verbascum L. (Scrophulariaceae) species namely, Verbascum bellum Hub.-Mor., Verbascum detersile Boiss. & Heldr., Verbascum myriocarpum Boiss. & Heldr. and Verbascum pestalozzae Boiss., growing in Turkey. Plant materials were extracted with chloroform, ethylacetate and methanol for antimicrobial tests. These extracts were assayed against both gram-positive and gram-negative bacteria by the microdilution method. The minimum inhibitory concentrations of the Verbascum species varied between 150-0.59 mg/ml. In general, ethylacetate extract was effective for E.coli (ATCC 25922, 1.88 mg/ml). Ethyl acetate extract of V. pestalozzae exhibited the highest effect on P. aeroginosa (ATCC 29853, 0.59 mg/ml). The antioxidant capacity of the studied species was only tested with methanol extracts. Their antioxidant action was tested by DPPH and β-carotene-linoleic acid methods. While V. pestalozzae (IC₅₀=15 µg/ml) exhibited the strongest activity in DPPH assay, V. detersile and V. pestalozzae provided an excellent inhibition effect (100% RAA) in the β-caroten- linoleic acid system. HPLC analysis of methanol extracts was also carried out to determine the composition of the phenolic compounds responsible for the activity. Methanol extracts were also subjected to HPLC analysis to determine their phenolic compound profile. Fri, 28 Oct 2011 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/19830 2011-10-28T00:00:00Z http://localhost:80/xmlui/handle/123456789/19829 Title: Comparative Synthetic Study of 1,4-Dihydroxy Anthraquinone (Quinizarin) Dye Intermediate Authors: KHALID PERVEZ BHATTI; MUHAMMAD ZUBER Abstract: 1,4-Dihydroxy anthraquinone (quinizarin) has been synthesised with two different precursors’ p-chlorophenol and hydroquinone by condensation of phthalic anhydride. It has been noticed that p-chlorophenol gave higher yield as compared to hydroquinone on reaction with phthalic anhydride at 200°C within 3:30 hours in a mixture of concentrated H₂SO₄ and boric acid. Three disperse dyes have been prepared from the quinizarin. The physical properties of three different colored dyes have also been discussed. Thu, 27 Oct 2011 00:00:00 GMT http://localhost:80/xmlui/handle/123456789/19829 2011-10-27T00:00:00Z