ENVIRONMENTAL STUDIES OF DIFFERENT EFFECTS OF LEAD ON SOME PHYSIOLOGICAL AND MORPHOLOGICAL FEATURES OF DIPTERA FLIES

Abstract

Lead is an important environmental toxic waste which almost contaminate the food, soil, water and air, hence, insects could be influenced easily by the lead. Therefore, lead was studied in the form of lead acetate using different doses. viz 0.125 mg., 0.25 mg., 0.5 mg., 1.0 mg and 2.0 mg, inrespect of its effects on external morphology of Bactrocera dorsalis, Bactrocera zonata, Bactrocera cucurbitae, Drosophila melanogaster and Musca domestica at 48 hours post treatment. It was observed that under the influenced of lead morphological abnormalities were developed in the larvae of flies. Morphological changes were observed as elongated wings, de-shaped wings, elongated and folded legs, change in color of larvae, pupae and adults, several other structural abnormalities of larvae and pupae shape were also observed. It is shown that Dipterous flies could present a useful module for the assessment of lead contamination. The effect of lead acetate on proteins of five species of dipterious flies Bactrocera dorsalis, Bactrocera zonata, Bactrocera cucurbitae, Drosophila melanogaster and Musca domestica were also observed through electrophoresis, while Egg albumin 42.7 kDa was used as a reference protein and various proteins of different weights were found altered.