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http://142.54.178.187:9060/xmlui/handle/123456789/11392
Title: | Isolation and Characterization of Cadmium Metallothionein Gene from Local Ciliates |
Authors: | Nighat, Rukhsana |
Keywords: | Natural Sciences |
Issue Date: | 2009 |
Publisher: | University of the Punjab Quiad-e-Azam Campus, Lahore 54590, Pakistan. |
Abstract: | First cadmium metallothionein like gene PMCd1 of a ciliate, Paramecium sp., isolated from indus- trial wastewater has been cloned and sequenced. PMCd1 is an intronless gene, encoding 612 nucleotides, with TAA coding for glutamine. The coding region of PMCd1 com- prises 203 amino acids, including 37 cysteine residues with a conserved structural pattern in the form of recurring structural motifs, arranged in 17 x-cys-x-y-cys-x, 1 x-cys- cys-x and x-cys-x contexts. Both, the deduced amino acids and nucleotide sequence differ, not only from other animal metallothioneins (MTs), but also from the previously characterized Tetrahymena Cu and Cd-MTs. The translated protein of PMCd1 contains conserved cysteine residues, peculiar characteristic of stress inducible metallothionein genes of ciliates and other groups of organisms. |
URI: | http://142.54.178.187:9060/xmlui/handle/123456789/11392 |
Appears in Collections: | Thesis |
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