Production of Amylolytic Enzymes for Industrial Use

Abstract

The present work describes the production of amylolytic enzymes such as alpha-amylase and amyl glucosidase by locally isolated cultures of Bacillus subtilis and Aspergillus Niger respectively. Wheat bran and brans of other cereals were evaluated as substrates for enzyme formation by both solid substrate and submerged fermentation methods. Of all substrates, however, wheat bran was found to be the best substrate for the production of enzymes. Optimum conditions for maximum enzyme production by both processes were determined. The solid substrate fermentation process was found to be more economical method for the synthesis of enzyme using locally available raw materials. Solid substrate fermentation was carried out in 1-liter conical flasks containing wheat bran or bran of other cereals as substrate. The use of wheat bran or rice bran for the production of enzymes by submerged fermentation process was carried out in liter conical flasks on rotary shaker and 50 1 glass stainless steel fermenter. Alpha-amylase of Bacillus subtilis in the crude form was evaluated as desizing for removal of starch from grey cloth in the textile industries. The results were quite comparable with the imported materials. Grey fabric more than 100,000 meters was desized. Two tons of maize starch was also hydrolyzed at the packages Ltd. For sizing paper during manufacturing process. The performance of starch hydrolysate as sizing was quite satisfactory and economical as compared with that of oxidized starch. Maize starch and defatted rice bran were also hydrolyzed to glucose by enzyme- enzyme process i.e., using both enzymes in the crude form.