Industrial Enzgnology and their Characterization

Abstract

The present work was undertaken to utilize the industrial waste material with (sugar cane baggage) Rice husk rice polishing and cake of oil seeds (castor bean and mooring oilers) in the development of enzymes. This plan underlines the use of indigenous materials to produce end cellular and exocellular industrial enzymes by giving microorganism. Phospholipase, lipases and protease, the industrial enzyme have been produced by growing penellicius expensium, using rice husk and the carbon source for the same organism. Penicillin expensium was obtained from the microbiology department, Glasgow University Scotland. This strain was selected primarily because no enzymatic study of such strain was repeated in the literature. Method of culture; stock culture of P. expensium was maintained on agar slants containing 2% Bactoagar, peptone and 2% dextrose. Spore suspension was obtained by adding sterilized water to a stock slant. Growth of P.expensium was carried out on Basel medium, containing 0.75% milled rice husk with, and without glucose solution sepratly sterilized in two separate flasks as the carbon source. The media were inoculated with 50*10 6 SPORES IN SUSPENSION AND WERE INCUBATED IN ORBITAL SHAKING INCUBATOR ADJUSTED AT 28% for five days. The test enzymes (lipolytic and protease) were examined period in both samples after every 24 hours of the incubation period. The phospholipase B, lipase and protease activates were found present in significant amounts in all the incubation samples.