In-vivo and in-vitro studies to investigate the anti-diabetic mechanisms underlying Moringa oleifera leaf ethanol extracts

Abstract

Obesity, insulin resistance and cardiovascular complications are endemic global health diseases due to ingestion of high carbohydrate. To prevent such health complications, this study was conducted to report another appealing option that can prevent postprandial hyperglycemia. For in vivo studies, 95% ethanol (v/v) extract of Moringa oleifera and its butanol fraction were given orally once a day for 14-days to streptozotocin-induced diabetic rats. The blood samples were collected to determine plasma insulin, monocyte chemoattractant protein-1 (MCP-1) and glucose-6phosphate (G6P) level. α-amylase and α-glucosidase enzyme kinetics against fixed concentrations of samples (100 µg/mL) was conducted. The M. oleifera ethanol (v/v) extract at 1000 and 500 mg/kg and butanol fraction at 500 mg/kg dose raised the level of G6P and lowered MCP-1 level significantly but failed to increase the level of insulin significantly. The results also indicated non-competitive inhibition of α-amylase, α-glucosidase and a significant increase in glucose uptake by rectus abdominus muscle.