Effects of ceftazidime with and without imipramine and bromazepam on behavior and neuro-inflammatory parameters in rats with chronic suppurative otitis

Abstract

The use of antibiotics in middle ear perforation due to chronic supprative otitis media (CSOM) is being widely employed across the world. The object of the present study was to evaluate the effect of ceftazidime with and without imipramine and bromazepam on behavior neuro-inflammatory parameters (serotonin, immunoglobulin and lymphotoxin beta) in CSOM induced rats. The rats were divided into five groups (six in each), G1; negative control (healthy rats maintained on normal saline), G2; positive control (diseased without any treatment), G3 (ceftazidime 15 mg/kg), G4 (ceftazidime 15 mg/kg with imipramine 1.15mg/kg) and G5 (ceftazidime 15 mg/kg with bromazepam 0.09mg/kg. All the drugs were administered intra-peritoneally for seven days. Behavior studies were conducted after treatment period and the serum was subjected for Elisa method for the estimation of inflammatory markers. Behavioral deficit and decreased serotonin and elevated IgE and LT-beta induced by CSOM was significantly reverted back to the normal levels in groups received imipramine or bromazepam with ceftazidime but not in group treated only with ceftazidiem. Therefore, the antidepressant or anxiolytic drugs may be helpful at low doses to attenuate the depression associated with chronic suppurative otitis media alongwith neuro-inflammatory markers (serotonin, IgE and LT-beta).