Quantification and characterization of Myo-inositol in locally available peanuts and pine nuts in Pakistan

Abstract

Inositol is one of nine biologically significant isomers of hexa hydroxy-cyclohexane which plays important role for bone formation and bone mineral density. The study was conducted to quantify and characterize the myo inositol crystals isolated from the pine nuts and peanuts. High-performance liquid chromatography, UV, FTIR and XRD was utilized for the rapid, on-line detection of inositol phosphate (InsP). The absorption peaks at 382.63nm for 0.10mg peanuts and 382.68 for 0.07mg pine nuts by UV-Visible spectroscopy confirmed the presence of myoinositol. Total concentration of myoinositol obtained was 0.23 and 5.31mmol/kg. The X-ray crystallography analysis showed that the unit-cell parameters a = 6.7226 (3) Å, b = 12.1462 (5) Å, c = 18.9942 (8) Å, α= 89.00, β= 94.98, δ= 989.00. The crystal density was reported at 1.6 cm−3 while the crystal volume was recorded at 1504.7(11) Å3. The stretching frequencies of myo-inositol crystals were observed in the region of 3800-3778 cm−1 related to alcohol O-H groups of myo-inositol. It is therefore concluded and recommended that dry fruits being the rich source of myo-inositol can be used as functional food for the treatment of polycystic ovarian syndrome.