Effect of Toddalia asiatica extract combined with miR-483 on proliferation, apoptosis and inflammatory factors expression of osteoarthritis chondrocyte

Abstract

This study aims to investigate the effect of Toddalia asiatica extract combined with miR-483 on osteoarthritis chondrocyte proliferation and apoptosis and expression of inflammatory factors. Osteoarthritis chondrocytes were treated with Toddalia asiatica extract. Osteoarthritis chondrocytes were transfected with anti-miR-483 or miR-483, and Toddalia asiatica extract was applied. The levels of TNF-α, IL-6, IL-10 were determined by ELISA. Cell proliferation and cloning were evaluated by MTT and cloning experiment. Cell apoptosis was determined by flow cytometry. P21 and caspase-3 protein expression were analyzed by Western blot. The expression of miR-483 was detected by qRT-PCR. The application of Toddalia asiatica extract or inhibition of miR-483 significantly increased the cell survival rate, cloning count and IL-10 level of osteoarthritis chondrocytes, and significantly reduced the apoptosis rate, levels of P21, Caspase3, TNF-α IL-6 and miR-483 expression level of osteoarthritis chondrocytes (p<0.05). Overexpression of miR-483 could reverse the promotion effect of Toddalia asiatica extract on osteoarthritis chondrocytes proliferation, clone formation and IL-10 level, as well as reserve the inhibition effect of Toddalia asiatica extract on osteoarthritis chondrocyte apoptosis and levels of P21, caspase-3, TNF-α, IL-6. The combination of Toddalia asiatica extract and miR-483 can promote the proliferation of osteoarthritis chondrocytes and inhibit apoptosis and expression of inflammatory factors.