Rat alpha glucosidase inhibitor and phytochemicals activities of endophytic actinobacteria from Ficus deltoidea

Abstract

Ficus deltoidea is a medicinal plant that has high endophytic actinobacteria diversity. Endophytic actinobacteria play an important role in producing various types of bioactive compounds including α-glucosidase inhibitor. Screening of 40 endophytic actinobacteria isolates from F. deltoidea showed that 77% of them had inhibitory activity against rat α-glucosidase. The 64% of isolates that have rat α-glucosidase inhibitor activity were derived from leaves. TBL 7, TBL 24, TBS 3, TBS 17 and TBR 20 have high activity. Based on the molecular identification of the 16S rRNA gene, five selected isolates have similarity with Streptomyces spp. The aqueous and n-hexane extracts of TBL 7 isolates had the lowest IC50 values of 159.25 µg/ml and 118.52 µg/ml, respectively. Qualitative phytochemical analysis showed that aqueous and n-hexane extracts of TBL 7 contained flavonoids, phenols, alkaloids, triterpenoids, tannins, and saponins. These results showed that endophytic actinobacteria from F. detoidea have the potential to be developed as αglucosidase inhibitor.