Please use this identifier to cite or link to this item: http://localhost:80/xmlui/handle/123456789/13194
Title: Production and antimicrobial activity of silver nanoparticles synthesized from indigenously isolated Pseudomonas aeruginosa from Rhizosphere
Authors: Arzoo, Shabana
Naqvi, Zoha
Hussain, Mushtaq
Shamim, Sumbul
Fatima Zeb, Tehseen
Ali, Shaukat
Keywords: Nanoparticles
Rhizosphere
Pseudomonas
silver
antimicrobial activity
Issue Date: 15-Nov-2020
Publisher: Karachi:Pakistan Journal of Pharmaceutical Sciences, university of Karachi.
Citation: Arzoo, S., Naqvi, Z., Hussain, M., Shamim, S., Zeb, T. F., & Ali, S. (2020). Production and antimicrobial activity of silver nanoparticles synthesized from indigenously isolated Pseudomonas aeruginosa from Rhizosphere. Pakistan Journal of Pharmaceutical Sciences, 33.
Abstract: Nanoparticles hold profound biological, pharmaceutical and industrial applications. Green synthesis of nanoparticles is considered amongst the environmentally safe and cost effective method of nanoparticle synthesis. Briefly, Pseudomonas strains were isolated from rhizosphere soil samples. Cell free supernatants of 36% of the isolates showed production of silver nanoparticles. Of these, three isolates, SMS13, SMS100 and SMS124 were selected as the potentially best nanoparticle producers. Amplification and DNA sequencing of 16srRNA gene identified all three strains as Pseudomonas aeruginosa. Scanning electron microscopy showed existence of particles between the ranges of 60nm to 70nm in the diameter with elemental composition of silver varies from 0.48% to 1.61%. Consistently, surface plasmon resonance showed maximum absorbance of nanoparticles between 352nm to 406nm. Finally, antibacterial activity of the synthesized nanoparticles was assessed by spot assay whereas Cell Free Supernatants (CFS) of respective isolates were taken as control against clinical isolates of Salmonella typhi, Shigella dysentriae, Klebsiella pneumoniae, P. aeruginosa, Proteus mirabilis and Streptococcus epidermidis. Nanoparticles suspension from all isolates exhibited antibacterial activity against all the screened isolates, whereas no biological activity was observed in the CFS of corresponding strains.
URI: http://142.54.178.187:9060/xmlui/handle/123456789/13194
ISSN: 1011-601X
Appears in Collections:Issue 6

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