PREVALENCE OF MASTITIS AND IN-VITRO ANTIBIOGRAM STUDY OF THE MASTITOGENS IN BHAG-NARI CATTLE

Abstract

Mastitis is a multifactorial disease of dairy cattle having multiple etiological agents and is considered to be the economically most costly disease of dairy animals worldwide. This study was accomplished to determine the prevalence and antibiogram against most common mastitogens in Bhag-Nari cattle breed in Balochistan province of Pakistan. For this, 323 Bhag-Nari cattle were screened for subclinical mastitis by Surf-Filed-Mastitis-Test (SFMT) while clinical mastitis was diagnosed by close examination of udder and milk. Milk samples from mastitis positive cattle were collected aseptically and were cultured for isolation and identification of S. aureus, Strep. agalactiae and Streptococcus dysaglactiae. Antibiotic sensitivity against mastitogens was tested on MullerHilton agar by disc diffusion method as per CSLI standards. Prevalence of subclinical and clinical mastitis was 13.62% and 2.16%, respectively. Underfed cows have higher prevalence (18.65%) than well-fed cows (5.63%). Likewise prevalence was significantly high (P<0.05; 16.90%) in cows with no practice of teat dipping compared to cows with teat dipping (7.69%). Cows milked once showed high prevalence (17.06%) than cows milked twice or more. Out of mastitis positive cases, prevalence of S. aureus, Str. agalactiae and and Str. Dysagalactiae was 50.98%, 29.41% and 19.60%, respectively. The antibiogram sensitivity tests showed that the S. aureus, Str. agalactiae and Str. dysagalctiea were sensitive to Ceftiofur, Oxytetracyclinc, chlortetracycline and Cephradine. It was concluded that Staphylococcus areus was the most prevalent mastitogens in Bhag-Nari cattle, and Ceftiofur, Oxytetracyclinc, chlortetracycline and Cephradine effective against S. aureus, Str. agalactiae and Str. dysagalctiae