Please use this identifier to cite or link to this item:
http://localhost:80/xmlui/handle/123456789/13467
Title: | Simvastatin inhibites high glucose-induced renal tubular epithelial cells apoptosis by down-regulating miR-92a |
Authors: | Guan, Ying Zhou, Ping Sun, Zhangsong Meng, Lifeng |
Keywords: | Simvastatin miR-92a renal tubular epithelial cells apoptosis |
Issue Date: | 16-Jan-2021 |
Publisher: | Karachi: Faculty of Pharmacy & Pharmaceutical Sciecnes, University of Karachi |
Abstract: | This work aims to investigate the role of simvastatin (SIM) in renal tubular epithelial cells (HK-2) proliferation. The apoptosis model of HK-2 cells induced by high glucose was established; HK-2 cells were cultured in vitro and randomly divided into control group, model group, SIM low-dose group, SIM medium-dose group and SIM high-dose group. After 24 h culture, the inhibitory effect of SIM on high glucose- induced proliferation of HK-2 cells was evaluated by MTT method. The expression of cysteinyl aspartate specific proteinase (Caspase-3) in apoptosisrelated protein was evaluated by Western blotting; miR-92a expression in HK-2 cells was measured by RT-qPCR. High glucose group had significantly lower HK-2 cell survival rate than the control group (p<0.05); SIM middle-dose and high-dose groups had higher HK-2 cell survival rate than the model group, (p<0.05); SIM low, medium and high-dose groups had lower HK-2 cell apoptosis rate, Caspase-3 protein and miR-92a expression levels than the model group (p<0.05), all showing dose-dependence. |
URI: | http://142.54.178.187:9060/xmlui/handle/123456789/13467 |
ISSN: | 1011-601X |
Appears in Collections: | Issue 01 (Special) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
6-8874-SP.htm | 140 B | HTML | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.