Please use this identifier to cite or link to this item: http://localhost:80/xmlui/handle/123456789/13467
Title: Simvastatin inhibites high glucose-induced renal tubular epithelial cells apoptosis by down-regulating miR-92a
Authors: Guan, Ying
Zhou, Ping
Sun, Zhangsong
Meng, Lifeng
Keywords: Simvastatin
miR-92a
renal tubular epithelial cells
apoptosis
Issue Date: 16-Jan-2021
Publisher: Karachi: Faculty of Pharmacy & Pharmaceutical Sciecnes, University of Karachi
Abstract: This work aims to investigate the role of simvastatin (SIM) in renal tubular epithelial cells (HK-2) proliferation. The apoptosis model of HK-2 cells induced by high glucose was established; HK-2 cells were cultured in vitro and randomly divided into control group, model group, SIM low-dose group, SIM medium-dose group and SIM high-dose group. After 24 h culture, the inhibitory effect of SIM on high glucose- induced proliferation of HK-2 cells was evaluated by MTT method. The expression of cysteinyl aspartate specific proteinase (Caspase-3) in apoptosisrelated protein was evaluated by Western blotting; miR-92a expression in HK-2 cells was measured by RT-qPCR. High glucose group had significantly lower HK-2 cell survival rate than the control group (p<0.05); SIM middle-dose and high-dose groups had higher HK-2 cell survival rate than the model group, (p<0.05); SIM low, medium and high-dose groups had lower HK-2 cell apoptosis rate, Caspase-3 protein and miR-92a expression levels than the model group (p<0.05), all showing dose-dependence.
URI: http://142.54.178.187:9060/xmlui/handle/123456789/13467
ISSN: 1011-601X
Appears in Collections:Issue 01 (Special)

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