ISOLATION AND CHARACTERIZATION OF LIPASE PRODUCING BACTERIA FROM TANNERY EFFLUENT

Abstract

Lipase are important hydrolytic enzymes that catalyze the hydrolysis of triacylglycerol to glycerol and free fatty acids. Microbial lipases are important extracellular enzymes because of their diverse applications in biotechnological and microbiological practice from many years. Lipase have been used for various industrial purposes and in the manufacture of many beneficial products. The main purpose of this study was to isolate and characterize the lipase producing bacteria from different samples collected from tannery effluents located in Kasur. Eight bacterial strains named as L1, L3, L5, L6, L9, L10, L11, L12 were isolated followed by screening on Tributyrin Agar Medium with Mustard oil. These bacterial strains were found to be gram positive cocci and rods. Five strains showed similarity with Bacillus spp. (L5, L6, L10, L11, L12) and three as Staphylococcus spp. (L1, L3, L9). The growth kinetic study revealed that these bacterial strains showed optimum growth at temperature 37° and pH 7. These bacterial strains were optimized for nutritional estimation of carbon sources and nitrogen sources. Sucrose and yeast extract were found to be best carbon and nitrogen source, respectively. This study revealed that tanneries can be the good source of lipase producing bacteria that can be used for potential industrial importance.