Please use this identifier to cite or link to this item: http://localhost:80/xmlui/handle/123456789/13574
Title: Antibacterial efficacy of silver nanoparticles against metallo-βlactamase (blaNDM, blaVIM, blaOXA) producing clinically isolated Pseudomonas aeruginosa
Authors: Qureshi, Rabia
Qamar, Muhammad Usman
Shafique, Muhammad
Muzammil, Saima
Rasool, Muhammad Hidayat
Ahmad, Ijaz
Ejaz, Hasan
Keywords: P. aeruginosa
MBL
MIC
silver nanoparticles
Issue Date: 16-Jan-2021
Publisher: Karachi: Faculty of Pharmacy & Pharmaceutical Sciecnes, University of Karachi
Abstract: Carbapenem resistance in Pseudomonas aeruginosa is a major concern in the public health sector, primarily in developing countries such as Pakistan. Therefore, novel approaches such as Silver nanoparticles (AgNPs) can be used to address emerging concerns. Clinical isolates (n=200) were reconfirmed using selective media and API 20NE kit. The antibiogram was determined according to the CLSI 2016 guidelines. Molecular detection was carried out by PCR. Antibacterial activity in AgNPs was achieved by dilution method. Of 200 P. aeruginosa, mostly (n=82; 41%) were isolated from pus samples. Of 110 MDR P. aeruginosa, 70 (63%) were carbapenemase and 58 (52%) were MBL producers. Antimicrobial profile of MBL producing P. aeruginosa reported that all isolates were resistant to β-lactams, and 89% to levofloxacin and ciprofloxacin except colistin. Of 25 (35.7%) blaNDM producing P. aeruginosa, 12 isolates (48%) had MIC 16μg/mL to imipenem. Of 23 (32%) blaVIM producing P. aeruginosa, 12 (52%) contained MIC 16μg/mL to imipenem. However, 12 (17.1%) blaOXA-48 producing P. aeruginosa, 4 (33%) contained MIC 16μg/mL to imipenem. In vitro AgNPs activity inhibited and killed MBL producing isolates at 1 mg/mL and 2 mg/mL, respectively. AgNPs may be used as an alternative therapy followed by multiple clinical trials.
URI: http://142.54.178.187:9060/xmlui/handle/123456789/13574
ISSN: 1011-601X
Appears in Collections:Issue 01 (Supplementary)

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