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Please use this identifier to cite or link to this item: http://142.54.178.187:9060/xmlui/handle/123456789/13580
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dc.contributor.authorArshad, Numera-
dc.contributor.authorIshtiaq, Saiqa-
dc.contributor.authorKhan, Farrakh Zia-
dc.contributor.authorDanish, Zeeshan-
dc.contributor.authorRashid, Ahmad Junaid-
dc.contributor.authorIjaz, Bushra-
dc.contributor.authorTariq, Somayya-
dc.date.accessioned2022-10-24T08:41:59Z-
dc.date.available2022-10-24T08:41:59Z-
dc.date.issued2021-01-16-
dc.identifier.issn1011-601X-
dc.identifier.urihttp://142.54.178.187:9060/xmlui/handle/123456789/13580-
dc.description.abstractStudy has been premeditated to appraise the anticancer and anti-inflammatory activities of a native medicinal plant Saussurea hypoleuca Spreng root. Anticancer assays including MTT, Alamar Blue (AB), Neutral Red (NR) & LDH were employed on root methanolic extract (RME) and all fractions to calculate % age of cell viability and cell cytotoxicity. All fractions of plant root were tested for in vitro as well as in vivo anti-inflammatory assays by reported methods. GC-MS analysis of n-hexane: chloroform fractions in column chromatography has shown isopropyl myristate, hexadecanoic acid, 11-octadecenoic acid, Di-n-octyl phthalate, dioctyl ether, decanedioic acid, 1H-3a,7-Methanoazulene, 3,4-hexanedione and Tetracosapentaene. Percentage of cell viability in anticancer assays was significantly high in all fractions. However, whole results were momentous with ethyl acetate and aqueous fractions owning to excellent profile in evaluating cytotoxicity in each assay. COX-2 inhibition was calculated which was high in RME (68.69%), ethyl acetate (56.52%), aqueous (55.21%) and chloroform fraction (53.47%). Carrageenan and formalin models were developed on rats to investigate in vivo anti-inflammatory activity. RME (56.19%, 71.09%, 66.4%, 67.99%) and ethyl acetate (51.36%, 64.97%, 55.63% & 61.01%) produced significant % age inhibition in dose dependent manner at 200 and 400 mg/kg doses respectively. All above findings direct that plant root holds strong anticancer and antiinflammatory activities.en_US
dc.language.isoenen_US
dc.publisherKarachi: Faculty of Pharmacy & Pharmaceutical Sciecnes, University of Karachien_US
dc.subjectAnticanceren_US
dc.subjectanti-inflammatoryen_US
dc.subjectGC-MSen_US
dc.subjectCOX-2en_US
dc.subjectMTTen_US
dc.subjectAlamar blueen_US
dc.subjectneutral reden_US
dc.subjectLDHen_US
dc.titleGC-MS analysis, anticancer and anti-inflammatory activities of Saussurea hypoleuca spreng. Rooten_US
dc.typeArticleen_US
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