Evaluation of wound healing effects of Syzygium cumini and laser treaqtment in diabetic rats

Abstract

This study was aimed to investigate the antioxidant effects of Syzygium cumini (S. cumini) seeds extract along with wound healing score in correlation to glutathione and blood glucose levels in diabetic rats. Sprague-Dawley rats were divided into 8 groups (n=6) and diabetes was induced by a single intraperitoneal injection of streptozotocin (40 mg kg-1 body weight). An alcoholic extract of S. cumini was administered to punch plier induced wounded rats and changes in serum glutathione levels, wound healing score and blood glucose levels were examined. Laser treatment of 500 mW for 5 minutes was given once a day for 12 days at 810 and 630 nm respectively. Statistical analysis was performed using one way ANOVA. A p-value <0.05 was considered significant and p-value <0.001 was considered highly significant. There was significant increase in glutathione levels and wound healing score when S. cumini extract was administered oral and topical along with topical laser therapy. There was significant reduction in the blood glucose levels upon administration of S. cumini extract in diabetic rats. This study concludes that there was a positive relation between the glutathione levels and wound healing score, since there was increase in wound healing score with the increase in the glutathione levels.