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Please use this identifier to cite or link to this item: http://142.54.178.187:9060/xmlui/handle/123456789/13807
Title: GC/MS analysis, anti-leishmanial and relaxant activity of essential oil of Chenopodium ambrosioides (L.) from Malakand region
Authors: Niaz Ali
Nabi, Muhammad
Shoaib, Mohammad
Shah, Ismail
Ahmed, Ghayour
Shakirullah, Shakirullah
Ziauddin, Ziauddin
Wadood Ali Shah, Syed
Ghias, Mehreen
Khan, Shahzeb
Keywords: Chenopodium ambrosioides
essential oil
anti-leishmanial
Leishmania tropica
antispasmodic
verapamil
calcium channel blocking
Issue Date: 12-Mar-2021
Publisher: Karachi:Pakistan Journal of Pharmaceutical Sciences, university of Karachi.
Citation: Ali, N., Nabi, M., Shoaib, M., Shah, I., Ahmed, G., Shah, S. W. A., ... & Ali, W. (2021). GC/MS analysis, anti-leishmanial and relaxant activity of essential oil of Chenopodium ambrosioides (L.) from Malakand region. Pakistan Journal of Pharmaceutical Sciences, 34(2).
Abstract: Chenopodium ambrosioides is abundantly available in Malakand region. As constituents and concentrations of essential oils vary based on its geographical location, we carried our current study to extract and evaluate its possible relaxant activity in rabbits’ jejunum and anti-leishmanial activity against promastigotes of Leishmania tropica. The essential oil was obtained from aerial fresh parts through steam distillation followed by GC/MS analysis. Antispasmodic activity was performed on spontaneous and KCl induced contractions. Curves for calcium concentration response (CCRCs) were prepared with and without different concentrations of essential oils and verapamil - a standard calcium channel blocker as per our reported procedures. GC/MS analysis indicated that the essential oil contains 4-carene (56.59%) and o-cymene (41.46%), the two most abundant compounds previously reported from this species. The LD50 value for acute toxicity is 279.66±2.2mg/kg. The essential oil have significant antileishmanial activity with LC50 of Log10 (1.83±0.0026) ×10-6mg/ml, potent relaxant activity on rabbits’ jejunal preparations with respective EC50 = 1.46±0.15mg/ml for spontaneous activity. For KCl (80mM) induced contractions, EC50=0.26±0.02mg/ml. In CCRCs, the oil produced a right shift as exhibited by verapamil. More, its relaxant activity, which is mediated through calcium channel blocking mechanism, proves a rationale for its traditional use in gut spasm.
URI: http://142.54.178.187:9060/xmlui/handle/123456789/13807
ISSN: 1011-601X
Appears in Collections:Issue 2

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