Effect of metformin combined with chemotherapeutic agents on gastric cancer cell line AGS

Abstract

To explore the effect of metformin combined with chemotherapeutic agents on gastric cancer cell line AGS, 24 patients with gastric cancer were tracked for treatment. CCK-8, Transwell model and flow cytometry were used to detect the cell proliferation, migration ability and other indexes. The metformin inhibited the AGS cell proliferation in a dose- and time-dependent manner (P<0.05). The application of metformin, cisplatin, adriamycin or paclitaxel alone could effectively lower the migration and invasion ability of AGS cells. The metformin in combination with the three chemotherapeutic agents could effectively promote the apoptosis of AGS cells. The metformin in combination with chemotherapeutic agents can effectively and apparently treat the patients with gastric cancer, more significantly in clinic compared to the traditional administration mode. It can effectively promote the apoptosis of AGS cells, thus, it’s worth adopting in clinic.