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  6. Pakistan Journal of Pharmaceutical Sciences
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  10. Issue 02
Please use this identifier to cite or link to this item: http://142.54.178.187:9060/xmlui/handle/123456789/14123
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dc.contributor.authorSALIMI, MONA-
dc.contributor.authorPOURABDI, LADAN-
dc.contributor.authorZAKERI, MARYAM-
dc.contributor.authorABDIPOUR, NAZANIN-
dc.date.accessioned2022-11-28T09:36:45Z-
dc.date.available2022-11-28T09:36:45Z-
dc.date.issued2009-07-05-
dc.identifier.citationSalimi, M., Pourabdi, L., Zakeri, M., & Abdipour, N. (2009). Separation of phenolic glycolipids in mycobacterium bovis BCG by reversed-phase high performance liquid chromatography. Pakistan Journal of Pharmaceutical Sciences, 22(2).en_US
dc.identifier.issn1011-601X-
dc.identifier.urihttp://142.54.178.187:9060/xmlui/handle/123456789/14123-
dc.description.abstractA crude phenolic glycolipid extract from Mycobacterium bovis BCG was fractionated by column chromatography. A reversed-phase high performance liquid chromatography (HPLC) method with UV detection at 275nm was developed for simultaneous detection and separation of phenolic glycolipids (PGLs) in Mycobacterium bovis BCG. This analysis provides a good resolution. Different solvent systems and columns for HPLC were compared. A system composed of acetonitrile-water in the ratio of 0→80% at a flow rate of 0.8 mL/min and C8 analytical column were found to be optimum for HPLC of the phenolic glycolipids. This simple method is therefore appropriate to purify these compounds present in M. bovis extract.en_US
dc.language.isoenen_US
dc.publisherKarachi: Faculty of Pharmacy & Pharmaceutical Sciences, Karachien_US
dc.subjectHPLC-UVen_US
dc.subjectmycobacteriumen_US
dc.subjectphenolic glycolipiden_US
dc.titleSEPARATION OF PHENOLIC GLYCOLIPIDS IN MYCOBACTERIUM BOVIS BCG BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHYen_US
dc.typeArticleen_US
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