QUANTITATIVE ESTIMATION OF β-SITOSTEROL, LUPEOL, QUERCETIN AND QUERCETIN GLYCOSIDES FROM LEAFLETS OF SOYMIDA FEBRIFUGA USING HPTLC TECHNIQUE

Abstract

Soymida febrifuga (Meliaceae) dried leaflets (10 gm) were extracted with petroleum ether. Unsaponifiable matter quantitatively used for sample preparation, labeled as SF-U. Another 10 gm leaflet powder was extracted with methanol and quantitatively used for sample preparation labeled as SF-A. Sample and standard solution were dosage on three different plates and developed in its respective mobile phase plates were scanned using TLC scanner III and estimated using integration software CATs 4.05. Calculations for percentage were done considering standard and sample Rf, AUC and dilution factor. Estimation of β Sitosterol, Lupeol, Quercetin, Quercetin-3-O-galactoside, Quercetin-3-O-xyloside and Quercetin-3-O-rutinoside were determined as 0.02146 % w/w, 0.0377 % w/w, 0.4079 % w/w, 0.6197 % w/w, 2.974 % w/w and 3.235 % w/w respectively with the help of HPLC techniques.