SPECTROPHOTOMETRIC AND SPECTROFLUORIMETRIC METHODS FOR DETERMINATION OF RACECADOTRIL

Abstract

Two accurate and sensitive spectrophotometric and spectrofluorimetric methods were developed for determination of Racecadotril. In the first method reduction of Fe3+ into Fe2+ in presence of o-phenanthroline by Racecadotril to form a stable orange-red ferroin chelate [Fe- (Phen)3] 2+ was the basis for its determination . The absorbance at 510 nm was measured and linear correlation was obtained in the concentration range of 2.5 – 25 µg mL-1. In the second method the native fluorescence of Racecadotril in acetonitrile solvent at λ = 319 nm when excitation was at 252 nm is used for its determination. Linear correlation was obtained in the concentration range of 50 to 500 ng mL-1. The proposed methods were applied for determination of Racecadotril in bulk powder with mean accuracy of 100.39±1.239 for the spectrophotometric method and 100.09± 1.042 for the spectrofluorimetric method. The proposed methods were successfully applied for determination of Racecadotril in its pharmaceutical dosage form.