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Please use this identifier to cite or link to this item: http://142.54.178.187:9060/xmlui/handle/123456789/14352
Title: OPTIMIZATION OF THE CULTURE CONDITIONS IN THE RICE (ORYZA SATIVA CV. BASMATI-370)
Authors: TAHIR, ROMESA
BATOOL, MARIA
MAHMOOD MINHAS, NASIR
ALI, SHAUKAT
MASOOD, SHAHID
ULLAH AJMAL, SAIF
Issue Date: 30-Apr-2012
Publisher: Karachi: Pakistan Journal of Botany, Botanical Garden, University of Karachi
Citation: Romesa, T., Maria, B., Minhas, N. M., Shaukat, A., Shahid, M., & Ajmal, S. U. (2012). Optimization of the culture conditions in the rice (Oryza sativa cv. Basmati-370). Pakistan Journal of Botany, 44(2), 671-673.
Abstract: The objective of the present study was to develop an efficient callus induction protocol for a fine rice (Oryza sativa L.) variety Basmati-370. Mature grains of this variety were used for callus induction. The Chu’s N6 medium was used as basal medium for callus induction and was supplemented with sucrose, Myo-inositol, Casein, agar (4 g L-1, 5 g L-1, 6 g L-1) and growth hormone 2,4-Dichlorophenoxy acetic acid in different concentrations of 2ppm and 2.5ppm. The genotype responded well for all the three concentrations of agar (4 g L-1, 5 g L-1, 6 g L-1) and two concentrations of 2,4-D (2ppm, 2.5ppm), however, the combination of 4 g L-1 agar with 2.5ppm 2,4-D gave excellent results with maximum callus induction of 93.42% followed by 4 g L-1 agar with 2ppm 2,4-D and 5 g L-1 with 2.5ppm 2,4-D which also gave better results with the observed callus induction of 92.75% and 91.11%, respectively.
URI: http://142.54.178.187:9060/xmlui/handle/123456789/14352
ISSN: 2070-3368
Appears in Collections:2006,Part-1

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