A RP-HPLC METHOD FOR THE ASSAY OF CEFPIROME AND ITS APPLICATION IN DRUG-METAL INTERACTION STUDIES

Abstract

An accurate, sensitive and least time consuming RP-HPLC method for the estimation of cefpirome in the presence of essential and trace metal has been developed and validated. Cefpirome was eluted from a B144A, OD-5-100, C18 (150 x 4.6 mm) column at room temperature with a mobile phase consisting of MeOH:H2O (15:85, % v/v) at a flow rate of 1 ml/minute, while UV detection was performed at 265 nm. The detection limit of cefpirome was 10 ng. Drug metal interaction studies were carried out at 37 oC to monitor the complexation of drug with metal ions. These studies were beneficial to determine the drug in therapeutic concentrations inside human body as well as its complexation with metal cations. The metals essential to human body like Mg(II), Ca(II), Cr(II), Mn(II), Fe (III), Co(II), Ni(II), Cu(II), Zn(II) & Cd(II) were in the form of chlorides. The carboxylic group of the dehydrothiazine ring has more binding capacity relative to other group that augments the drug complexes with essential and trace elements. The established HPLC method is rapid, accurate, and selective, because of its sensitivity and reproducibility. The order of complexation was ferric > chromium > copper > nickel > cadmium > zinc > magnesium > manganese > calcium > cobalt.