DSpace logo

Please use this identifier to cite or link to this item: http://142.54.178.187:9060/xmlui/handle/123456789/14651
Full metadata record
DC FieldValueLanguage
dc.contributor.authorQamar, Muhammad Usman-
dc.contributor.authorMustafa, Ghulam-
dc.contributor.authorQaisar, Uzma-
dc.contributor.authorAzeem, Farrukh-
dc.contributor.authorShahid, Muhammad-
dc.contributor.authorManzoor, Irfan-
dc.contributor.authorQasim, Muhammad-
dc.contributor.authorAbbas, Tanveer-
dc.contributor.authorShah, Asad Ali-
dc.date.accessioned2022-12-05T07:59:17Z-
dc.date.available2022-12-05T07:59:17Z-
dc.date.issued2019-09-13-
dc.identifier.citationQamar, M. U., Mustafa, G., Qaisar, U., Azeem, F., Shahid, M., Manzoor, I., ... & Shah, A. A. (2019). Molecular detection of blaNDM and blaVIM in clinically isolated multi-drug resistant Escherichia coli in Pakistan. Pakistan Journal of Pharmaceutical Sciences, 32(S5), 2305-2309.en_US
dc.identifier.issn1011-601X-
dc.identifier.urihttp://142.54.178.187:9060/xmlui/handle/123456789/14651-
dc.description.abstractMetallo-β-lactamase (MBL) producing Escherichia coli are an emerging and serious threat to public health sector around the globe. MBL are spreading via plasmids to the host pathogens and produce resistance against carbapenems and left limited or no treatment option. Therefore, we designed this study to determine the dissemination of MBL producing E. coli in our locality. E. coli (n=100) were collected from various clinical samples from different tertiary care hospitals, Faisalabad. Microbes were sub-cultured on MacConkey and UTI Chromo Select agar. Bacteria were identified on the basis of culture characteristics and biochemically confirmed by API 20E. Antimicrobial susceptibility testing, carbapenemase and MBL was performed as per CLSI 2018 guidelines. Molecula r identification of MBL genes were performed using specific primers by PCR. Of 100 E. coli, majority of them isolated from urine (n=55) followed by pus (n=23) and blood (n=22). Antibiogram displayed that all the E. coli were resistant to β-lactam drugs including carbapenems followed by 76% to ciprofloxacin and 60% to amikacin. Among these, 81% were MBL producers. Molecular characterization revealed that 18.4% were blaNDM and 15.3% were blaVIM producers. This study concluded that there is high prevalence of MBL producing E. coli in our clinical settings.en_US
dc.language.isoenen_US
dc.publisherKarachi: Faculty of Pharmacy & Pharmaceutical Sciences, Karachien_US
dc.subjectEscherichia colien_US
dc.subjectmetallo-β-lactamaseen_US
dc.subjectNew Delhi metallo-β-lactamaseen_US
dc.subjectverona imipenemaseen_US
dc.titleMolecular detection of blaNDM and blaVIM in clinically isolated multidrug resistant Escherichia coli in Pakistanen_US
dc.typeArticleen_US
Appears in Collections:Issue 5 (Supplementary)

Files in This Item:
File Description SizeFormat 
14-SUP-1168.htm148 BHTMLView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.