Study on the wound healing, anti-inflammation and anti-bacterial activities of Jinjianling cream: A Chinese herbal compound

Abstract

The aim of the present study was the healing effect and anti-inflammatory effect of Jinjianling cream on skin lesions and to investigate the antibacterial activity in vitro, which proved that the preparation is safe and effective. The mouse scald model was established to observe the wound healing time and wound healing rate of mice, serum levels of TNF-α and IL-1 were measured by the ELISA method. The model of eczema in mice was induced by DNCB, and the degree of ear swelling in mice was calculated. The hematoxylin-eosin (HE) staining was used to make pathological sections and count inflammatory cells, and the change of serum IL-2 level was determined by the ELISA method. The bacteriostasis rate was determined by pour plate method, the diameter of inhibition zone (DIZ) was determined by filter paper diffusion method and the minimum inhibitory concentration (MIC) was determined by double dilution method. After treatment, the effect of Jinjianling cream groups on the healing of damaged skin in scalded mice was significant. The serum levels of TNF-a and IL-1 decreased, which were lower than those in the model group (p<0.05, p<0.01). In the mouse eczema model, the degree of ear swelling improved significantly, serum IL-2 level was decreased, and inflammatory cell count was significantly than the model group (p<0.05, p<0.01). The results of antibacterial experiments showed that bacteriostasis rate was positively correlated with drug concentration. DIZ values of bacteriostatic circle on Staphylococcus aureus and Escherichia coli were 17.25mm and 25.62mm. Moreover, the MIC values of two kinds of bacteria all were 64μg/mL. Jinjianling cream can promote the healing ability of damaged skin and reduce inflammation of the wound. It also has a strong inhibitory effect on wound pathogenic bacteria, can significantly improve wound healing and effectively treat dermatitis, eczema and other skin diseases.