EFFECT OF GENOTYPE AND INFLORESCENCE DEVELOPMENTAL STAGE ON CALLUS INDUCTION AND PLANT REGENERATION OF MISCANTHUS LUTARIORIPARIU, A NON-FOOD BIOMASS CROP

Abstract

Miscanthus lutarioriparius, is a perennial C4 weeds. In this study, tissue culture responses were evaluated in four genotyoes of M. lutarioriparius which collected from the Yangtze River basin. A total of four types of callus with different characteristics and induction frequencies were induced from four genotypes immature inflorescences on medium Murashige and Skoog (MS) containing 4.0 mg/L 2,4-dichlorophenoxyacetic acid (2, 4-D). Only ML04 generated the embryogenic callus. According to the length of immature inflorescences, they divided into three inflorescences developmental stages which generated different induction percentages of embryogenic callus. Developmental stage B with 50-100 mm length inflorescence generated the highest induction percentages of embryogenic callus. The regeneration rate was 65.56% (4.0 mg/L kinetin), 91.50% (5.0 mg/L KT) and 26.67% (6.0 mg/L KT) from embryogenic callus, respectively. The shoots were transferred onto the Murashige and Skoog (MS) basal medium for rooting, the rooting rate was 100%.