Relevant effects of Taohong Siwu decoction on isolated rat aortic ring dependent on endothelium nitric oxide-cGMP pathway

Abstract

Using rat thoracic aortic rings to test the relaxing effects of the 95% ethanol extract and aqueous extract of Taohong Siwu decoction (THSW) on endothelium intact or endothelium removed aortic rings. Results showed that the 95% ethanol extract (0.1, 1, 10, 100, 1000 mg·L-1 ) and aqueous extract (0.1, 1, 10, 100, 1000 mg·L-1 ) of THSW were able to relax the intact endothelium aortic rings pre-contracted by 10-6 mol·L-1 PE. 10-4 mol·L-1 L-NAME and 10-5 mol·L-1 methylene blue both were able to inhibit the relaxation other than indomethacin. For the endothelium removed aortic rings, potassium channel blocker 3×10-3mol·L-1 tetraethylammonium chloride and 10-5 mol·L-1 glibenclamide had no effect on the relaxation effects caused by the 95% ethanol extract and aqueous extract of THSW. It could be concluded that the 95% ethanol extract and aqueous extract of THSW relax blood vessel by endothelium-dependent way