Dermocosmetic emulgels for anti-aging effects: Evidence from chromatographic and non-invasive biophysical techniques

Abstract

Persimmon Fruits (Diospyros kaki L.f, Ebenaceae) and its active principles have long been used in traditional medicines for various cosmetics and skin conditions, however clinical efficacy on various facial skin parameters like roughness, scaliness, hydration, elasticity and wrinkles have not yet been reported. Current study was aimed to analyse polyphenolic constituents of Diospyros kaki fruit extract (DKFE) and to clinically evaluate dermocosmetic emulgels loaded with bioactive phytoconstituents from persimmon fruits, using non-invasive in-vivo evaluation techniques. HPLC analysis established the presence of quercetin, gallic acid, chlorogenic acid, ferulic acid, p-coumeric acid, catechin and cinnamic acid. Results revealed that test formulation produced significant and control showed insignificant (p>0.05) effects on moisture contents and elasticity. Surface evaluation of living skin (SELS) index values were reduced significantly (p≤0.05) for the emulgels loaded with DKFE, represented by reduction in skin wrinkles (-12.40%), roughness (-11.76%) and scaliness (-18.59%). Conclusively, a safe and compatible dermocosmetic emulgel formulation loaded with antioxidant enriched DKFE, revealed promising anti-aging attributes that may be due to presence of vital polyphenolic constituents as presented by HPLC analysis.