Please use this identifier to cite or link to this item: http://localhost:80/xmlui/handle/123456789/15212
Title: Ethanolic extract of Nigella sativa protects Fe(II) induced lipid peroxidation in rat’s brain, kidney and liver homogenates
Authors: Hassan, Waseem
Noreen, Hamsa
Ullah Khalil, Shafqat
Hussain, Arshad
Rehman, Shakilla
Sajjad, Shagufta
Rahman, Ata Ur
BT da Rocha, Joao
Keywords: Nigella sativa
lipid peroxidation
antimicrobial activities
Issue Date: 20-Jan-2016
Publisher: Karachi:Pakistan Journal of Pharmaceutical Sciences, university of Karachi.
Citation: Hassan, W., Noreen, H., Khalil, S. U., Hussain, A., Rehman, S., Sajjad, S., ... & da Rocha, J. B. (2016). Ethanolic extract of Nigella sativa protects Fe (II) induced lipid peroxidation in rat's brain, kidney and liver homogenates. Pakistan Journal of Pharmaceutical Sciences, 29(1).
Abstract: The study describes the effect of ethanolic extract of Nigella sativa against Fe(II) induced lipid peroxidation. Basal and Fe(II) induced thiobarbituric acid reactive species (TBARS) production was significantly inhibited by the ethanolic extract of Nigella sativa at 25-200µg/ml. Our data revealed that the extract has high DPPH radical scavenging activity at highest tested concentrations. The extract significantly chelated Fe(II) and scavenged hydroxyl (OH●) radical at 25-200µg/ml concentration. The nutritional analysis was performed and carbohydrate, fats, fiber, protein, moisture and ash content were measured in the studied extract. The phytochemical analysis confirmed the presence of alkaloid, carbohydrate & sugar, glycosides, phenolic compounds, flavonoids, protein and amino acid, phytosterols, tannins, gum and mucilage. The extract also showed significant antimicrobial activities against 10 bacterial strains i.e. Salmonella typhi, Bacillus subtilis, Bacillus cereus, Klebsiella pneumonia, Escheria coli, Xanthomonas, Salmonella heidelberg, Staphylococcus aureus, Clostridium and Escheria coli (human) and 5 fungal strains i.e. Aspergillus niger, Entomola, Aspergillus flavus, Alternaria alternata and Penicillium. This study confirms the potential antioxidant and antimicrobial activities of ethanolic extract of Nigella sativa which can be considered not only as a diet supplement but can be used against a variety of free radical induced damage diseases
URI: http://142.54.178.187:9060/xmlui/handle/123456789/15212
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