Please use this identifier to cite or link to this item: http://localhost:80/xmlui/handle/123456789/15240
Title: Effect of the serum of rats treated with Suo Quan pill on embryonic stem cells
Authors: Xu, Jing- Jing
Huang, Ping
Xiang, Yun-Ya
Tan, Bo
Wu, Qing-He
Ze-Hui Qin, Hong-Ying Cao
Keywords: Suo Quan pill
embryonic stem cells
serologic pharmacological method
Chinese medicine
Issue Date: 4-Jan-2018
Publisher: Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi
Citation: Xu, J. J., Huang, P., Xiang, Y. Y., Tan, B., Wu, Q. H., Qin, Z. H., & Cao, H. Y. (2018). Effect of the serum of rats treated with Suo Quan pill on embryonic stem cells. Pakistan Journal of Pharmaceutical Sciences, 31.
Abstract: Suo Quan pill(SQP), a well-known and classical traditional Chinese medicine compound, consists of three traditional Chinese medicine: Alpinia oxyphylla Miq., Lindera aggregata (Sims) Kosterm., Dioscorea opposite. Its effect was summarized as supplementing kidney- yang and shrinkaging urination. This study evaluated the effects of the serum of rats treated with Suo Quan pill on embryonic stem cells(ES cells). Cell proliferation was detected by MTT assay. Cell cycle and apoptosis of ES cells were evaluated with flow cytometry. Nanog mRNA expression was verified by fluorescence quantitative PCR and Nanog protein in ES cells was determined by Western blot. The serum of SQP-treated rats not only promoted ES cells proliferation and Nanog expression in ES cells, but also inhibited H202 stimulated cell apoptosis. Furthermore, the serum of rats containing SQP affected the cell cycle distribution of ES cells, reducing the percentage of cells in G0/G1phase and increasing the percentage of cells in G2/M phase, increasing the proliferation index of ES cells. These results illustrate that the enhanced effect of SQP on ES cells proliferation is in part due to the increased expression of Nanog in ES cells, the accelerated cell cycle period and the inhibited apoptosis of ES cells.
URI: http://142.54.178.187:9060/xmlui/handle/123456789/15240
ISSN: 1011-601X
Appears in Collections:Issue No.1 (Supplementary)

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