A novel spectrophotometric determination of caroverine in pharmaceutical formulations via derivatization with Folin-ciocalteu Phenol reagent

Abstract

In this study we have reported a new, fast and extraction free spectrophotometric procedure for the assessment of caroverine in pharmaceutical raw and tablet dosage forms. In the reported visible spectrophotometric procedure tungstate in Folin-Ciocalteu phenol reagent is reduced in alkaline medium and produces the blue colored chromogen that shows λmax at 740nm with the calibration range of 2-28µg/ml. The LOD and LOQ values are 1.15 and 3.81µg/ml respectively. The newly developed analytical procedure is used to determine caroverine in raw material of and commercial tablets dosage forms. The spectrophotometric method represented in this study is simple, rapid and extraction free. It may easily be utilized for the determination of caroverine in pharmaceutical laboratories for quality control and stability studies purpose.