Please use this identifier to cite or link to this item: http://localhost:80/xmlui/handle/123456789/15354
Title: A PRIMARY STUDY OF HIGH PERFORMANCE TRANSGENIC RICE THROUGH MAIZE UBI-1 PROMOTER FUSING SELECTIVE MAKER GENE
Authors: JINJUAN SHEN
PINGZHONG CAI
FENG QING
ZHIYONG ZHANG
GUIXUE WANG
Issue Date: 20-Apr-2012
Publisher: Karachi: Pakistan Botanical Society, University of Karachi
Citation: Shen, J., Cai, P., Qing, F., Zhang, Z., & Wang, G. (2012). A primary study of high performance transgenic rice through maize Ubi-1 promoter fusing selective maker gene. Pakistan Journal of Botany, 44(2), 501-506.
Abstract: Based on the expression vector pBI121, we successfully constructed a plant overexpression vector of Hspa4 gene fusing with selective maker gene (hygromycin-resistance gene) driven by the Ubi-1 promoter (pBI121-Ubi-Hpt-Hspa4, p121UHH). The plant expression vectors p121UHH and pCAMBIA1301-Ubi-Hspa4 (p1301UH) were transformed into the rice callus, mediated by Agrobacterium tumefaciens. We screened 17 p121UHH-positive transgenic plants and 15 p1301UH-positive transgenic plants by the hygromycin-resistance gene. The pick-up rate of the resistance callus was 51.7% and 42.5%, respectively, and the rate of regeneration for the resistance callus was 51.2% and 49.1%, respectively. The result of polymerase chain reaction (PCR) identification indicated that the pick-up rate of positive transgenic plants was 51.7% and 42.5% and the total transformation efficiency was 16.5% and 6.2%, and the former was 2.66 times of the later. The results of the experiment indicate that the possibility of the appearance of false positive results in the fusing of a plant overexpression vector with a selective maker gene is much less
URI: http://142.54.178.187:9060/xmlui/handle/123456789/15354
ISSN: 2070-3368
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